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目的:分析结直肠腺瘤血清蛋白质谱的变化,寻找结直肠腺瘤的特异性生物标志物。方法:采用SELDI-TOF-MS技术(表面增强激光解析电离飞行时间质谱)对比分析31例结直肠腺瘤患者和11例正常人的血清蛋白质谱,用Biomarker Wizard软件对获得的蛋白质谱进行分析。结果:结直肠腺瘤组与正常对照组有24个蛋白峰有差异,其中有三个蛋白峰(8565.84D、8694.51D和5910.50D)的差异非常显著,8565.84D和8694.51D在结直肠腺瘤中高表达,在正常人中低表达,而5910.50D在两组人群中的表达相反。结论:这三个蛋白峰可能为结直肠腺瘤特异性的生物蛋白标志物。
Objective: To analyze the changes of serum protein profiles in colorectal adenomas and to search for specific biomarkers of colorectal adenomas. METHODS: Serum protein profiles of 31 patients with colorectal adenoma and 11 normal controls were analyzed by SELDI-TOF-MS (surface enhanced laser desorption ionization time-of-flight mass spectrometry). Biomarker Wizard software was used to analyze the protein profiles. RESULTS: There were 24 protein peaks in colorectal adenoma group and normal control group, among which there were three protein peaks (8565.84D, 8694.51D and 5910.50D), the difference was very significant, 8565.84D and 8694.51D in colorectal adenoma Expression, low expression in normal people, and 5910.50D in the two groups of people the opposite expression. Conclusion: The three protein peaks may be specific biomarkers for colorectal adenomas.