论文部分内容阅读
用[~3H]-3-氧-甲基-D-葡萄糖摄取的方法测定细胞水含量,观察谷氨酸受体激动剂、拮抗剂对培养的星形神经胶质细胞水含量的影响,并观察细胞内、外钙的作用.结果发现:0.5,1,10 mmol/L的谷氨酸和1mmol/L的trans-ACPD(代谢型谷氨酸受体激动剂)1h均可以引起细胞的水含量增加,1mmol/L的 AMPA(离子型谷氨酸受体激动剂)不影响细胞的水含量,1mmol/L的L-AP3(代谢型谷氨酸受体拮抗剂)可以拮抗1mmol/L谷氨酸和trans-ACPD的作用;撤除细胞外液的钙,谷氨酸不再引起细胞的水含量增加, 20 μmol/ L 的 CdCl_2不能减轻谷氨酸的作用,而300μmol/L的CdCl_2及30μmol/L的胆罗啉(Dantrolene)均可以减轻谷氨酸的作用,提示代谢型谷氨酸受体激动引起星形细胞肿胀,细胞内、外Ca~(2+)在谷氨酸引起的星形细胞肿胀中起一定的作用.
The cell water content was measured by uptake of [~ 3H] -3-oxo-methyl-D-glucose and the effects of glutamate receptor agonists and antagonists on the water content of cultured astrocytes were observed. The results showed that 0.5,1,10 mmol / L glutamic acid and 1 mmol / L trans-ACPD (metabotropic glutamate receptor agonist) could induce cellular water 1mmol / L AMPA (ionotropic glutamate receptor agonist) did not affect the water content of cells, 1mmol / L L-AP3 (metabotropic glutamate receptor antagonist) can antagonize the 1mmol / L Valley Glutamic acid no longer caused the increase of water content in cells, 20 μmol / L CdCl 2 could not relieve the effect of glutamate, while 300 μmol / L CdCl 2 and 30 μmol / L of choleroline (Dantrolene) can reduce the role of glutamate, suggesting that activation of metabotropic glutamate receptors cause astrocyte swelling, intracellular and extracellular Ca2 + in glutamate-induced star Cell swelling plays a role.