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目的:本研究通过建立糖尿病大鼠动物模型,观察海藻溴酚化合物A、B对糖尿病大鼠机体抗氧化水平的影响。方法:采用STZ注射法制作糖尿病(DM)大鼠模型,随机分为空白对照组、糖尿病模型组、化合物A低剂量组及高剂量组、化合物B低剂量组及高剂量组,灌胃给药12周。12周末处死大鼠,测肾匀浆中谷胱甘肽过氧物酶(GSH-Px)的活力及丙二醛(MDA)的含量;并采用透射电镜观察大鼠肾组织的病理改变。结果:与空白对照组相比,糖尿病模型组肾组织匀浆中GSH-Px活力下降,MDA含量升高,差异有统计学意义(P<0.05)。各干预组中GSH-Px的活力较糖尿病模型组有升高的趋势,MDA含量有下降趋势。电镜下各干预组肾小球及肾小管病变较糖尿病组减轻,且高剂量组优于低剂量组。结论:溴酚化合物A、B能提高糖尿病大鼠机体抗氧化水平,并能一定程度的改善肾脏病理改化,但其具体机制有待进一步探讨。
OBJECTIVE: In this study, we established animal models of diabetic rats and observed the effect of bromophenols A and B on the anti-oxidation of diabetic rats. Methods: Diabetic (DM) rats were induced by STZ injection and were randomly divided into blank control group, diabetic model group, low dose and high dose Compound A group, low dose and low dose Compound B group, 12 weeks. The rats were sacrificed on the 12th week to measure the activity of glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in the kidney homogenate. The pathological changes of the rat kidney were observed by transmission electron microscopy. Results: Compared with the blank control group, the activity of GSH-Px in renal tissue homogenate of diabetic model group decreased and MDA content increased (P <0.05). The activity of GSH-Px in each intervention group tended to increase compared with the diabetic model group, and the MDA content tended to decrease. Under electron microscope, glomerular and renal tubular lesion in each intervention group was alleviated compared with diabetic group, and the high dose group was better than the low dose group. CONCLUSION: Bromophenol compounds A and B can improve the antioxidant status of diabetic rats and can improve renal pathological changes to a certain extent, but the specific mechanism remains to be further explored.