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目的探讨贴敷预处理对随后佐剂性关节炎(AA)大鼠炎症的影响。方法应用福氏完全佐剂制作AA动物模型,24只Wistar大鼠随机分为正常组、AA组和预贴敷组(自制中药膜剂贴敷大鼠大椎穴),观察3组大鼠关节局部炎症和血清IL-1、IL-6、TNF-α含量。结果预贴敷组、AA组大鼠的足肿胀率和炎性因子与正常组有显著性差异;与AA组相比,预贴敷组大鼠足肿胀程度明显减轻(P<0.01),TNF-α含量有下降趋势,IL-1和IL-6含量下降(P<0.01和P<0.05),而且HE染色关节滑膜及软骨炎细胞浸润、血管增生及软骨破坏亦明显减轻。结论贴敷预处理能减轻随后AA大鼠早期炎症反应和关节病理损害程度,具有一定抵抗随后疾病损伤的保护作用。
Objective To investigate the effect of patch pretreatment on inflammation of rats with adjuvant arthritis (AA). Methods AA animal model was established by Freund’s complete adjuvant, and 24 Wistar rats were randomly divided into normal group, AA group and pre-adhering group (made homemade Chinese herbal film applied to Dazhui(Dazhui)) and the joints of the three groups were observed. Inflammation and serum IL-1, IL-6, TNF-α content. Results The foot swelling rate and inflammatory factors in the pre-adhered group and the AA group were significantly different from those in the normal group. Compared with the AA group, the paw swelling in the pre-adhered group was significantly reduced (P<0.01). -α content decreased, IL-1 and IL-6 levels decreased (P<0.01 and P<0.05), and HE-stained synovium and cartilage cell infiltration, vascular proliferation and cartilage destruction were also significantly reduced. Conclusion The patch pretreatment can reduce the degree of early inflammatory reaction and joint pathological damage in subsequent AA rats, and it has certain protection against subsequent disease damage.