论文部分内容阅读
目的琼脂糖等电聚焦结合双抗体过氧化物酶标记、亲和素-生物素放大技术检测CSF寡克隆区带,研究该方法的敏感性和特异性。方法琼脂糖等电聚焦结合双抗体过氧化物酶标记、亲和素-生物素放大技术检测21例多发性硬化(MS)患者、42例神经系统炎性疾病(NID)患者和19例神经系统非炎性疾病(NNID)患者的CSF及对照血清。结果MS患者寡克隆区带阳性率为47．6％,MS组与NID组、NNID组的差异均有统计学意义(P<0．0125)。结论该方法敏感,是MS临床诊断有价值的免疫学指标。我国MS患者寡克隆区带的阳性率较欧美地区低,但与亚洲一些国家及我国的台湾和香港地区接近,阳性率差异可能与东西方MS患者的免疫遗传背景不同有关。
Objective agarose isoelectric focusing combined with double antibody peroxidase markers, avidin - biotin amplification technology to detect CSF oligoclonal bands to study the sensitivity and specificity of the method. Methods Twenty - one patients with multiple sclerosis (MS), 42 patients with nervous system inflammatory disease (NID) and 19 patients with nervous system were detected by agarose isoelectric focusing combined with double antibody peroxidase and avidin - biotin amplification. Non-inflammatory disease (NNID) patients with CSF and control serum. Results The positive rate of oligoclonal band in MS patients was 47.6%. There was significant difference between MS group and NID group and NNID group (P <0.0125). Conclusion The method is sensitive and is a valuable immunological indicator of clinical diagnosis of MS. The positive rate of oligoclonal band in MS patients in China is lower than that in Europe and the United States, but it is close to some Asian countries in Asia and Taiwan and Hong Kong in China. The difference in positive rate may be related to the different immune genetic backgrounds of MS patients in East and West.