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目的研究含非甲基化CpG基序的免疫刺激寡核苷酸(CpG-ODN)联合重组HBsAg对慢性乙肝(CHB)患者外周血树突状细胞(dendriticcell,DC)表面标志、功能及胞浆信号传导与转录激活因子(STAT)、细胞信号转导抑制因子(SOCS)表达的影响。方法由CHB患者和健康者外周血单核细胞诱导扩增DC,以CpG2ODN或联合HBsAg刺激DC,并与TNF2α比较,评价其对DC表面标志、分泌IL212p70及刺激同种T细胞增殖能力的影响;应用Westernblot法检测DC胞浆STAT1、3、4、5、6以及SOCS1、3蛋白的表达。结果与PBS组比,TNF2α、CpG-ODN或联合HBsAg能明显提高CHB患者DC表面分子HLA-DR表达,IL-12p70分泌增加,刺激同种T细胞增殖能力增强,CpG-ODN联合HBsAg还能明显提高CD1a的表达;CpG-ODN或联合HBsAg、TNF2α对DC胞浆STAT1、4、6和SOCS1、3的表达呈不同程度增强作用,对STAT3、5的表达无明显影响。结论CpG-ODN与TNF-α一样能促进CHB外周血DC分化、成熟;CpG2ODN或联合HBsAg能增强DC的特异性抗原提呈作用;其作用机制可能是通过调节DC细胞内STAT1、4、6以及SOCS1、3等信号蛋白的表达。
Objective To investigate the effects of CpG-ODN combined with recombinant HBsAg on the surface markers, functions and cytoplasm of peripheral blood dendritic cells (DCs) in patients with chronic hepatitis B (CHB) Effects of signal transducers and activators of transcription (STAT) and cell signal transduction inhibitory factor (SOCS) expression. Methods DCs were induced from peripheral blood mononuclear cells of CHB patients and healthy donors. DCs were stimulated with CpG2 ODN or combined with HBsAg and compared with TNF2 α to evaluate the effects of DC on surface markers, secretion of IL212p70 and proliferation of allogeneic T cells. Western blotting was used to detect the expression of STAT1, 3, 4, 5, 6 and SOCS1, 3 in DCs. Results Compared with PBS group, TNF-α, CpG-ODN and HBsAg could significantly increase the expression of HLA-DR on DCs, increase the secretion of IL-12p70 and enhance the proliferation of allogeneic T cells. CpG-ODN and HBsAg were also significantly increased Improve the expression of CD1a; CpG-ODN or combined with HBsAg and TNF2α enhanced the expression of STAT1, 4,6 and SOCS1,3 in DC to varying degrees, but had no obvious effect on the expression of STAT3,5. Conclusions CpG-ODN, like TNF-α, can promote DC differentiation and maturation in peripheral blood of CHB patients. CpG2 ODN or combined HBsAg can enhance the specific antigen-presenting function of DC. Its mechanism may be through regulating STAT1, 4, 6 and SOCS1, 3 and other signaling proteins.