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目的:探讨垂盆草总黄酮及异鼠李素对对乙酰氨基酚(APAP)诱导的人正常肝细胞(L02)损伤的保护作用。方法:以不同浓度的APAP刺激L02细胞,制备损伤模型,筛选APAP的有效刺激浓度;复制APAP诱导的L02细胞损伤模型,用细胞增殖与活性试剂(CCK-8)检测细胞存活能力,分析盆草总黄酮及异鼠李素对APAP诱导的L02细胞活性的影响;检测细胞上清液中丙二醛(MDA),谷胱甘肽(GSH)含量,超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px)活性,丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的含量。结果:与正常组比较,不同浓度的APAP处理的L02细胞活力显著降低,筛选出的造模条件为10 mmol·L~(-1)APAP孵育24 h,模型组肝细胞上清液中MDA含量升高(P<005,P<001),GSH含量降低,SOD,GSH-Px活性降低,AST和ALT含量升高;与模型组比较,垂盆草总黄酮、异鼠李素均能显著提高APAP损伤细胞的存活率,降低MDA含量,提高GSH含量,提高SOD,GSH-Px活性,降低ALT和AST的释放量(P<005,P<001)。结论:垂盆草总黄酮及异鼠李素对APAP损伤的L02细胞具有较好的保护作用。
OBJECTIVE: To investigate the protective effects of total flavonoids of Rhizoma Imperatae and isorhamnetin on paracetamol (AP02)-induced normal human hepatocytes (L02) injury. METHODS: L02 cells were stimulated with different concentrations of APAP to prepare the injury model, and the effective stimulation concentration of APAP was screened; the model of L02 cell injury induced by APAP was duplicated, and cell viability was tested by cell proliferation and activity reagent (CCK-8). Effects of total flavonoids and isorhamnetin on APAP-induced L02 cell activity; detection of malondialdehyde (MDA), glutathione (GSH) content, superoxide dismutase (SOD), and glutathione in cell supernatants Glycoperoxidase (GSH-Px) activity, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) content. RESULTS: Compared with the normal group, the activity of L02 cells treated with different concentrations of APAP was significantly reduced. The selected model conditions were 10 mmol·L -1 APAP and incubated for 24 h. The content of MDA in the hepatocyte supernatant of the model group was determined. Increased (P<005, P<001), GSH content decreased, SOD, GSH-Px activity decreased, AST and ALT levels increased; compared with the model group, S. chinensis total flavone, isorhamnetin can significantly improve the APAP injury The survival rate of cells decreased the content of MDA, increased the content of GSH, increased the activities of SOD and GSH-Px, and reduced the release of ALT and AST (P<005, P<001). Conclusion: The total flavonoids and isorhamnetin of S. chinensis have a good protective effect on AP02-injured L02 cells.