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目的:建立HPLC法同时测定不同产地莪术中莪术二酮、莪术醇、吉玛酮、呋喃二烯、β-榄香烯等5种倍半萜类成分的含量。方法:采用Kromasil C18柱(4.6mm×250mm,5μm);流动相:乙腈(A)-水(B),梯度洗脱(0～35min,45%A→70%A;35～40min,70%A→90%A;40～60min,90%A→95%A),流速1.0mL·min-1;检测波长214nm;柱温25℃。结果:莪术二酮、莪术醇、吉玛酮、呋喃二烯、β-榄香烯分别在27.88～278.8,20.42～204.2,3.475～34.75,5.380～53.80,10.12～101.2μg·mL-1范围内线性关系良好,r≥0.999 6。平均加样回收率(RSD)分别为97.36%(1.85%),97.96%(2.30%),97.47%(2.02%),98.10%(2.38%),98.94%(2.01%)。结论:本方法操作简便、准确可靠,适用于莪术中5种倍半萜类成分的定量分析。
OBJECTIVE: To establish an HPLC method for the simultaneous determination of 5 sesquiterpenoids from Curzane, Curcuma longa, Jimaprone, Furodiene and β-Elemene in different areas. Methods: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used. The mobile phase consisted of acetonitrile (A) -water (B) A → 90% A; 40-60 min, 90% A → 95% A) at a flow rate of 1.0 mL · min -1; detection wavelength was 214 nm; Results: Curdione, curcumol, jimaprone, furadiene and β-elemene were in the range of 27.88-278.8, 20.42-204.2, 3.475-34.75, 5.380-53.8, 10.12-101.2 μg · mL-1, respectively Linearity is good, r≥0.999 6. The average recoveries (RSDs) were 97.36% (1.85%), 97.96% (2.30%), 97.47% (2.02%), 98.10% (2.38%) and 98.94% (2.01%), respectively. Conclusion: The method is simple, accurate and reliable and is suitable for the quantitative analysis of five sesquiterpenoids in Curcuma.