论文部分内容阅读
目的:以现代制剂技术提取中药的有效成分,对提取制备工艺进行考察,制备清瘟退热颗粒剂,并建立清瘟退热颗粒的质量标准。方法:以煎煮法对处方药材进行提取,用湿法制粒的制备工艺制备颗粒,用薄层色谱的方法鉴别柴胡、白芍、黄芩和大青叶,采用HPLC法测定清瘟退热颗粒中黄芩苷的含量。结果:薄层鉴别斑点清晰,阴性对照无干扰。黄芩苷的峰面积和进样量在0.194~1.164μg范围内,有良好的线性关系(r=0.999 5),平均加样回收率为100.89%,RSD为0.74%(n=6)。结论:提取方法及制备工艺成熟可靠,薄层鉴别及含量测定方法能够对该颗粒进行准确、快速定性、定量分析,结果稳定,可靠,重复性好,可用于该颗粒的质量控制。
OBJECTIVE: To extract the active ingredients of traditional Chinese medicine with modern preparation technology, investigate the extraction and preparation process, prepare the Qingwenjiedu granules, and establish the quality standard of Qingwenjieji granules. Methods: The prescription herbs were extracted by decocting method, the granules were prepared by wet granulation process, the Bupleurum root, Radix Paeoniae Rubra, Radix Scutellariae and Folium Isatidis were identified by TLC. The content of baicalin. Results: TLC identification spots clear, negative control without interference. The peak area of baicalin and injection volume in the range of 0.194 ~ 1.164μg, a good linear relationship (r = 0.999 5), the average recovery was 100.89%, RSD was 0.74% (n = 6). Conclusion: The extraction method and preparation technology are mature and reliable. The method of TLC and content determination can accurately, rapidly and qualitatively and quantitatively analyze the pellets. The results are stable, reliable and reproducible, and can be used for the quality control of pellets.