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目的探索甲醛对脱细胞DNA的影响,并初步建立脱细胞-核DNA检测加合物的新模型。方法用染毒缓冲液配制浓度分别为4%、1%、0.25%和0%的甲醛,用羟自由基损伤的脱细胞-核DNA作为检测加合物的试验模型,每组6张脱细胞-核DNA板,用彗星实验检测各组脱细胞-核DNA损伤情况,组间差异用SPSS 11.0软件统计分析。结果甲醛染毒的DNA损伤顺序为:0%=0.25%>1%组>4%,呈现明显的剂量-反应关系。结论甲醛可直接和脱细胞DNA片段形成加合物和/或DNA-DNA交联,脱细胞-核DNA模型可用于DNA加合物和/或交联物的检测。
Objective To explore the effect of formaldehyde on acellular DNA and to establish a new model of detection of adducts by acellular-nuclear DNA. Methods Formaldehyde (4%, 1%, 0.25%, and 0%) was prepared by using the poisoning buffer. The decellularized nuclear DNA damaged by hydroxyl radical was used as the experimental model for detecting adduct. Six decellularized cells - Nuclear DNA panels, using a comet assay to detect each group of acellular - nuclear DNA damage, differences between groups using SPSS 11.0 software statistical analysis. Results Formaldehyde exposure DNA damage sequence: 0% = 0.25%> 1%> 4%, showing a significant dose-response relationship. Conclusion Formaldehyde can directly form adduct and / or DNA-DNA cross-links with acellular DNA fragments. The decellularization-nuclear DNA model can be used for the detection of DNA adducts and / or cross-linkers.