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通过建立体外肿瘤坏死因子(TNF-α)诱导人脐静脉血管内皮细胞(HUVEC)炎症损伤反应模型,研究桂花多酚纯化组分1、2、3对炎症细胞的活性(MTT)、活性氧(ROS)含量、超氧化物歧化酶(SOD)和黄嘌呤氧化酶(XOD)活性影响;以10μg/L TNF-α诱导HUVEC产生炎症反应,加入6000μg/L、3000μg/L、300μg/L和30μg/L桂花多酚纯化组分1、2、3,研究桂花多酚纯化组分1、2、3的浓度及其组成对炎症细胞保护作用的影响。结果表明,当桂花多酚纯化组分2、3浓度为300~3000μg/L、组分1浓度为3000μg/L时,可显著提高由TNF-α诱导的HUVEC细胞活力和SOD酶活性(p<0.05),显著抑制炎症细胞中活性氧(ROS)含量和XOD酶活性(p<0.05),具有显著抗炎效果。
The inflammatory response model of human umbilical vein endothelial cells (HUVEC) was induced by the establishment of tumor necrosis factor (TNF-α) in vitro, and the activity of inflammatory components (MTT), reactive oxygen species (ROS), superoxide dismutase (SOD) and xanthine oxidase (XOD) activity were measured. HUVECs were induced to induce inflammation by 10μg / L TNF-α. 6000μg / L, 3000μg / L, 300μg / L and 30μg / L osmanthus polyphenols purification components 1, 2, 3, to study the concentration of purified components of osmanthus polyphenols 1, 2 and 3 and its composition on the protective effect of inflammatory cells. The results showed that the activity of SOD and the activity of SOD in TNF-α-induced HUVECs were significantly increased when the concentration of purified fractions 2 and 3 of osmanthus polyphenol was 300 ~ 3000μg / L and the concentration of component 1 was 3000μg / L (p < 0.05), significantly inhibited the activity of reactive oxygen species (ROS) and XOD activity (p <0.05) in inflammatory cells, and had a significant anti-inflammatory effect.