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目的观察反式白藜芦醇对阿尔茨海默病(Alzheimer’s disease,AD)模型小鼠记忆损伤的改善作用。方法小鼠随机分为假手术组、模型组和反式白藜芦醇10,20,40 mg·kg-1组。在小鼠海马CA1区注射Aβ25-35后给予反式白藜芦醇10 d,采用水迷宫试验观察药物对小鼠学习记忆的影响,免疫组化法观察各组小鼠海马CA1区神经元可塑性变化,Western-blot法检测神经可塑性相关蛋白的表达变化。结果 40 mg·kg-1反式白藜芦醇能明显缩短AD小鼠寻找平台的潜伏期,增加原平台所在象限的停留时间和穿越次数;20,40 mg·kg-1反式白藜芦醇能增加海马CA1区神经元顶端树突的长度和树突的密度;40 mg·kg-1反式白藜芦醇能增加AD小鼠海马CA1区BDNF、p CREB以及c-fos蛋白的表达。结论反式白藜芦醇能逆转Aβ引起的小鼠的学习记忆损伤,其机制可能与改善海马神经元的神经可塑性有关。
Objective To observe the effect of trans-resveratrol on memory impairment in Alzheimer’s disease (AD) model mice. Methods Mice were randomly divided into sham operation group, model group and trans-resveratrol 10,20,40 mg · kg-1 group. In the hippocampal CA1 area of mice injected with Aβ25-35 after trans-resveratrol given 10 d, water maze test to observe the effects of drugs on learning and memory in mice, immunohistochemical method to observe the hippocampal CA1 neuronal plasticity Changes, Western-blot detection of neuroplasticity related protein expression changes. Results Trans-resveratrol (40 mg · kg-1) significantly shortened the incubation period and the number of crossover times in the quadrupole of AD mice, while 20,40 mg · kg-1 trans-resveratrol Can increase the density of dendrites and the density of dendrites in the apical neurons of hippocampal CA1 area; and the trans-resveratrol at 40 mg · kg-1 can increase the expression of BDNF, p CREB and c-fos in hippocampal CA1 area of AD mice. Conclusion Trans-resveratrol can reverse the learning and memory impairment caused by Aβ in mice, and its mechanism may be related to the improvement of neuroplasticity in hippocampal neurons.