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目的通过考察低温胁迫后新疆紫草悬浮细胞次生代谢物量的变化,探讨道地产区特有的环境因子对新疆紫草的作用。方法 4℃低温胁迫新疆紫草悬浮细胞24h后,HPLC法分别测定胁迫前(计为0h)和胁迫后12、24、48、72、168h5个时间点迷迭香酸、紫草酸B、紫草呋喃A、紫草呋喃E、乙酰紫草素、脱氧紫草素、异丁酰紫草素、β,β’-二甲基丙烯酰紫草素、异戊酰紫草素和总紫草素类化合物的量。结果低温胁迫后不同时间点同类化合物的变化趋势基本一致,但其量差异显著,除β,β’-二甲基丙烯酰紫草素外,其他化学成分量的最大值均出现在低温胁迫组。结论低温胁迫可以促进新疆紫草悬浮细胞积累次生代谢物,并对揭示新疆紫草道地性形成的机制有重要作用。
Objective To investigate the changes of the secondary metabolites of Arnebia euchroma (Royle) Johnston seedlings after chilling stress and to explore the effect of the specific environmental factors on the shrubs of Lithocarpus. Methods After the cells were resuspended at 4 ℃ for 24 h, the contents of rosmarinic acid, lithospermate B, lithospermum, Furan A, comfrey furan E, acetylshikonin, deoxyshikonin, isobutyryl shikonin, β, β’-dimethylacrylshikonin, isovaleryl shikonin and total shikonin The amount of compound. Results The trend of similar compounds at different time points was basically the same after low temperature stress, but the difference was significant. The maximum values of other chemical components except β, β’-dimethylacrylshikonin appeared in the low temperature stress group . Conclusion Low temperature stress can promote the accumulation of secondary metabolites of Lithospermum cells in Xinjiang, and play an important role in revealing the mechanism of Lithospermum terrestris.