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将大豆种子凝集素基因的启动子(lec)从pBI-lec载体上酶切下来,连接到植物表达载体pCAMBIA3301(p3301)的多克隆位点上,然后运用RT-PCR方法扩增了拟南芥AtLACS9基因的编码区cDNA,并将其克隆到改造后的p3301载体的lec启动子之后,为进一步转化大豆、获得油份含量提高的转基因大豆做准备。结果表明:p3301载体改造成功,命名为p3301-lec;AtLACS9基因的编码区cDNA由2076 bp组成,编码含691个氨基酸残基的蛋白,其种子特异性表达载体p3301-lec-AtLACS9构建成功,可进行后续研究。
The lectin gene promoter of lectin (lec) was digested from the pBI-lec vector and ligated to the multiple cloning site of the plant expression vector pCAMBIA3301 (p3301). Arabidopsis thaliana AtLACS9 gene coding region cDNA, and cloned into the lec promoter of the transformed p3301 vector, in order to further transform the soybean, to obtain oil content increased transgenic soybean preparation. The results showed that the p3301 vector was successfully transformed and named as p3301-lec. The coding region of AtLACS9 gene consisted of 2076 bp and encoded a protein with 691 amino acid residues. The seed-specific expression vector p3301-lec-AtLACS9 was constructed successfully, Follow-up study.