论文部分内容阅读
目的:探讨RNA干扰技术沉默STAT3基因调控腺样囊性癌细胞生长的实验研究。方法:人腺样囊性癌生长细胞株ACC 2常规培养,分别采用A组为空白转染组,B组转染阴性对照siRNA(NC),C组转染特异性的siRNA(siRNA组)转染进ACC2,采用qRT PCR和Western blot法检测转染后STAT3 mRNA和蛋白表达水平的变化。MTT检测转染后腺样囊性癌细胞增殖。结果:ACC2细胞转染STAT3 siRNA 48 h后,STAT3 mRNA和蛋白的表达均受到明显抑制(P<0.05)。ACC 2生长72 h细胞转染后细胞增殖受到抑制,较对照组有显著性差异(P<0.05)。结论:STAT3基因有可能成为腺样囊性癌细胞生长基因治疗中重要的靶基因。
OBJECTIVE: To investigate the effect of STAT3 gene silencing on the growth of adenoid cystic carcinoma cells by RNA interference. Methods: Human adenoid cystic carcinoma cell line ACC 2 was cultured in a routine manner. A group was blank transfected group, B group transfected with negative control siRNA (NC), C group transfected with specific siRNA (siRNA group) Stained into ACC2, and the changes of STAT3 mRNA and protein expression after transfection were detected by qRT PCR and Western blot. The proliferation of adenoid cystic carcinoma cells was detected by MTT. Results: After STAT2 siRNA was transfected into ACC2 cells for 48 h, STAT3 mRNA and protein expression were significantly inhibited (P <0.05). Cell proliferation was inhibited after ACC 2 cells were transfected for 72 h, which was significantly different from that of control group (P <0.05). Conclusion: The STAT3 gene may be an important target gene in the gene therapy of adenoid cystic carcinoma cell growth.