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目的:观察不同浓度异常黑胆质成熟剂(ASMq)对体外培养的人增生性瘢痕成纤维细胞(HSFBs)转化生长因子-βl(TGF-β1)蛋白及m RNA表达的影响,在分子水平上探讨其抑制增生性瘢痕生长的作用机制。方法:体外培养人HSFBs,选取第3-5代细胞采用含不同浓度异常黑胆质成熟剂(200、400、600μg/m L)的DMEM培养液培养48h,运用RT-PCR技术检测各组TGF-β1 m RNA表达水平。运用Western bolt方法测量TGF-β1蛋白表达水平。结果:与对照组相比,3个药物剂量组HSFBs TGF-β1蛋白及m RNA的表达均减少(P<0.05),药物浓度为400μg/m L时效果最佳,且TGF-β1蛋白及m RNA表达的变化具有一致性。结论:ASMq有抑制体外培养的人HSFBs TGF-β1表达的作用,初步探讨了ASMq发挥抑制增生性瘢痕生长的作用机制,为ASMq药物的开发利用提供理论依据。
OBJECTIVE: To observe the effects of different concentrations of abnormal savda (ASMq) on the protein and mRNA expression of TGF-β1 in human hypertrophic scar fibroblasts (HSFBs) cultured in vitro. At the molecular level To investigate its mechanism of inhibiting the growth of hypertrophic scars. Methods: Human HSFBs were cultured in vitro. Cells of passage 3-5 were cultured in DMEM containing different concentrations of savda (200, 400, 600 μg / mL) for 48 h. RT-PCR was used to detect the expression of TGF -β1 m RNA expression level. Western bolt method was used to measure TGF-β1 protein expression. Results: Compared with the control group, the expressions of TGF-β1 protein and m RNA in HSFBs were all decreased (P <0.05), and the best results were obtained at the concentration of 400 μg / Changes in RNA expression are consistent. CONCLUSION: ASMq can inhibit the expression of TGF-β1 in human HSFBs cultured in vitro and preliminarily investigate the mechanism of ASMq in inhibiting the growth of hypertrophic scars, providing a theoretical basis for the development and utilization of ASMq drugs.