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目的建立用不加校正因子的主成分自身对照的简单高效液相色谱方法。方法采用美国月旭公司的UltimateTM C8色谱柱,规格(250mm×4.6mm,μm);流动相为:磷酸盐缓冲液(磷酸二氢钾6.8g和辛烷磺酸钠1g加水溶解并稀释至1000ml,用磷酸调节pH至3.3):5乙腈:甲醇(8∶1∶1),检测波长:240nm;进样量:10μL,流量:1.0mL·min-1;温度30℃。结果氨磺甲酯与其他杂质峰分离效果理想,检测结果准确。结论本测定方法简便快速,灵敏度高,专属性好,可用于氨磺甲酯中有关物质的检测。
Objective To establish a simple high performance liquid chromatography (HPLC) method using the principal component self-control without correction factor. The method uses the UltimateTM C8 column of the American Yue Xu company, the standard (250mm × 4.6mm, μm); the mobile phase is: phosphate buffer solution (potassium dihydrogen phosphate 6.8g and sodium octane sulfonate 1g dissolved in water and diluted to 1000ml , Adjusted to pH 3.3 with phosphoric acid: 5 acetonitrile: methanol (8: 1: 1), detection wavelength: 240 nm; injection volume: 10 μL, flow rate: 1.0 mL · min-1; Results Sulfomethionate and other impurities peak separation results are satisfactory, the test results are accurate. Conclusion The method is simple, rapid, sensitive and specific. It can be used to detect the related substances in sulfamethoxydine.