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目的 :研究治喘贴对卵清蛋白所致的哮喘实验大鼠体内T细胞亚群增殖及白介素 - 2 (1L - 2 )的调节作用。方法 :将实验大鼠随机分为对照组、哮喘模型组、代温灸膏治疗组、治喘贴治疗组 ,后者经外贴治疗 18d。采集大鼠胸腺及心脏血 ,采用流式免疫荧光技术和酶联免疫吸附 (ELISA)法分析其体内T细胞亚群增殖和血清中IL - 2的含量。结果 :治喘贴组和对照组大鼠胸腺细胞 (CD4+ 、CD8+ )S期、G2 +M期明显高于模型组 ,而细胞G0 /G1期低于模型组 (P均 <0 .0 5 ) ;治喘贴组大鼠血清中IL - 2含量明显高于对照组和模型组 (P均<0 .0 5 )。结论 :治喘贴能促进哮喘大鼠胸腺细胞 (CD4+ 、CD8+ )增殖 ,提高IL - 2的含量 ,从而调节机体免疫功能 ,改善气道变态性炎症反应。
Objective: To study the effect of Zhichuan Paste on the regulation of T lymphocyte subsets and interleukin - 2 (1L - 2) in asthmatic rats induced by ovalbumin. Methods: The experimental rats were randomly divided into control group, asthma model group, generation moxibustion ointment group and Zhichuan Paste group. The latter group received external treatment for 18 days. The thymus and heart blood of rats were collected. The proliferation of T lymphocyte subsets and IL - 2 in serum were detected by flow cytometry and enzyme - linked immunosorbent assay (ELISA). Results: The S phase and G2 + M phase of thymocyte (CD4 +, CD8 +) of Zhichuan paste group and control group were significantly higher than that of model group, while the G0 / G1 phase of model group was lower than that of model group (P <0.05) The content of IL - 2 in the Zhichuan paste group was significantly higher than that in the control group and model group (all P <0.05). Conclusion: Zhichuan can promote the proliferation of thymocytes (CD4 +, CD8 +) in asthmatic rats and increase the content of IL - 2, thereby regulating the immune function and improving the allergic airway inflammation.