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为了明确芳香植物对茶尺蠖Ectropisobliqua的驱避、拒食作用和获得应用于茶园茶尺蠖“推-拉”策略的植物材料,利用“Y”型嗅觉仪室内测定其成虫对芳香植物气味的趋性反应,并采用叶碟法测定芳香植物提取液对其幼虫的拒食作用及营养干扰效果。丁香罗勒、迷迭香、柠檬桉和芸香植株挥发物及甲醇提取液对茶尺蠖雌、雄成虫有显著的驱避效果。猫薄荷、迷迭香和鼠尾草的2 00 mg/mL甲醇提取液对茶尺蠖3龄幼虫表现出显著拒食活性,24 h后的选择性拒食率分别为93.85%、86.00%和77.32%,非选择性拒食率分别为85.77%、87.00%和88.05%。其中,迷迭香处理的幼虫生长率最低,仅为0.12 mg/天,对幼虫的相对取食量、食物利用率和食物转化率等营养指标有影响。研究表明,10种芳香植物中迷迭香具有显著的行为调控功能,是构建茶尺蠖“推-拉”策略的理想材料。
In order to clarify the repellent and antifeedant effect of aromatic plants on Ectropis obliqua and to obtain the plant material used in the tea garden tea plantation, the “Y” type olfactometer was used to determine the odor of adult plants against aromatic plants The anti-feeding effect of aromatic plant extracts on their larvae and the effect of nutrition interference were determined by leaf disc method. Clove basil, rosemary, lemon eucalyptus and rue plant volatiles and methanol extract had significant repellent effects on the female and male adults. The 200 mg / mL methanol extracts of catnip, rosemary and sage exhibited significant antifeedant activity against the 3rd instar larvae of C. rufipogon. The selective antifeedant rates after 24 h were 93.85%, 86.00% and 77.32%, respectively. Non-selective antifeedant rates were 85.77%, 87.00% and 88.05% respectively. Among them, the growth rate of larvae treated by rosemary was the lowest, only 0.12 mg / day, which affected the nutritional indexes of larvae such as relative food intake, food utilization and food conversion. Studies have shown that rosemary in 10 kinds of aromatic plants has a significant behavioral control function, and is an ideal material for building tea plantation push-pull strategy.