D-ribose is a unique, 5-carbon sugar that occurs naturally in all living cells. D-ribose has been used as a staring material for preparation of some certain medicines or just as a new nutraceutical that helps the body naturally restore its energy level. The fermentative processes are the best for their relatively simple series of production steps and relatively cheap starting materials. However, because of the many impurities in glucose yeast water and its dark color, pretreatment and purification must be done to the fluid before D-ribose can be separated. In this paper, separation and purification of D-ribose was carried out using cation exchange resins, of which Ca2+ exchange resin had the best effect. The optimal condition for adsorption is a flow velocity of 0.5BV/h at adsorption temperature of 25℃. The optimal condition for desorption is a flow velocity of 0.5 BV/h at desorption temperature of 80℃. D-ribose is a unique, 5-carbon sugar that arises naturally in all living cells. D-ribose has been used as a staring material for preparation of some certain medicines or just as a new nutraceutical that helps the body restore its energy level . The fermentative processes are the best for their relatively simple series of production steps and relatively cheap starting materials. However, because of the many impurities in glucose yeast water and its dark color, pretreatment and purification must be done to the fluid before D-ribose In this paper, separation and purification of D-ribose was carried out using cation exchange resins, of which Ca2 + exchange resin had the best effect. The optimal condition for adsorption is a flow velocity of 0.5BV / h at adsorption temperature of 25 ° C. The optimal condition for desorption is a flow velocity of 0.5 BV / h at desorption temperature of 80 ° C.