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目的 探讨类风湿性关节炎 (RA)患者血清抗Ⅱ型胶原 (CⅡ )抗体阳性的意义。方法 分别以人Ⅱ型胶原蛋白(HCⅡ )及牛Ⅱ型胶原蛋白CⅡ (BCⅡ )作为包被抗原进行间接ELISA法 ,检测 30例RA患者 ,对照组 (30例非RA的风湿病患者和 34例健康人 )血清抗CⅡ抗体。结果 以HCⅡ及BCⅡ作为包被抗原检测RA患者血清抗CⅡ抗体的阳性率分别约为 30 .0 %和 33.3% ;对照组血清中抗体阳性率均约为 1.6 % ;二者差异极其显著 (P <0 .0 1)。抗CⅡ抗体阳性的RA患者RF的阳性率高于抗CⅡ抗体阴性的RA患者。与HCⅡ和BCⅡ均发生阳性反应的血清 8例 ,均为早期RA患者。结论 抗CⅡ抗体可作为判断RA患者病情的辅助手段之一 ,牛CⅡ可替代人CⅡ进行有关RA发病机制中B细胞免疫的实验研究
Objective To investigate the significance of serum anti-type Ⅱ collagen (CⅡ) antibodies in patients with rheumatoid arthritis (RA). Methods Serum samples of 30 patients with rheumatoid arthritis (RA) and 30 patients with rheumatoid arthritis (RA) and 34 patients with rheumatoid arthritis (RA) were enrolled in this study. Healthy people) serum anti-C Ⅱ antibody. Results The positive rates of serum anti-CⅡ antibody in RA patients with HC Ⅱ and BCⅡ as coating antigen were about 30.0% and 33.3%, respectively. The positive rate of antibody in serum of control group was about 1.6%. The difference was significant (P <0 .0 1). The positive rate of RF in patients with anti-CII antibody-positive RA was higher than that in patients with anti-CII antibody-negative RA. Serum samples were positive for both HCⅡ and BCⅡ, all of which were early stage RA patients. Conclusion Anti-CⅡ antibody can be used as an adjunct to judge the condition of RA patients. CⅡ can substitute for human CⅡ to study the B cell immunity in the pathogenesis of RA