大麦黄花叶病毒(Barley yellow mosaic virus,BaYMV)广泛分布于西欧、日本和我国长江中下游和东部沿海地区,严重危害了大麦生产,并呈现出不断蔓延扩大的趋势。该病毒经土壤禾谷多粘菌介体感染大麦后,病毒繁殖发病的状况,易受到大麦品种不同、环境温湿度等因素的影响,发病程度的差异很大,而且还发现带毒隐症的大麦品种,使大麦抗源筛选和大麦抗性品种的选育工作受到严重干扰,因此迫切需要建立一种快速、灵敏和准确的检测方法。目前,比较理想的方法是ELISA等免疫学技术。然而 Barley yellow mosaic virus (Barley yellow mosaic virus, BaYMV) is widely distributed in Western Europe, Japan and China’s coastal areas in the middle and lower reaches of the Yangtze River and the eastern coast, which seriously endangers the production of barley and shows a trend of continuous expansion and expansion. The virus infected by soil cereal polymyxin mediator of barley, the incidence of virus replication, susceptible to different barley varieties, environmental temperature and humidity and other factors, the incidence of a great difference, but also found with poisoning Barley varieties have severely disrupted the screening of barley sources and breeding of barley varieties. Therefore, there is an urgent need to establish a rapid, sensitive and accurate detection method. At present, the ideal method is ELISA and other immunological techniques. however