为提高林荫银莲花(Anemone flaccida Fr.Schmidt)不定芽繁殖系数,对其组培快繁体系进行优化。以林荫银莲花无菌苗为外植体,研究了培养基中激素、碳源、添加物和矿质元素钙等组分对其不定芽增殖和生长状况的影响,得出最佳的不定芽增殖培养基配方。以1/2 Ca浓度MS为固体培养基,激素为1.0 mg/L 6-BA和1.0 mg/L NAA,蔗糖或食用白糖30 g/L,水解酪蛋白为添加物,浓度为750 mg/L,培养30 d后繁殖系数可达4.01,大幅提高了不定芽的增殖速度和质量,为工厂化生产林荫银莲花种苗奠定了基础。 In order to improve the multiplication coefficient of adventitious buds of Anemone flaccida Fr.Schmidt, its tissue culture system was optimized. The effects of hormone, carbon source, additive and calcium in the medium on the proliferation and growth of adventitious buds were studied using aseptic seedlings of Anemones Linn. As explants, and the best adventitious buds proliferation Base recipe. With 1/2 Ca concentration as solid medium, the hormones were 1.0 mg / L 6-BA and 1.0 mg / L NAA, sucrose or edible sugar 30 g / L, hydrolyzed casein as additive and the concentration 750 mg / L After 30 days of culture, the coefficient of multiplication was up to 4.01, which greatly enhanced the proliferation rate and quality of adventitious buds, which laid the foundation for the industrialized production of Linyin Anemone seedlings.