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目的观察氢醌能否延缓苯肼诱发的急性溶血性贫血的恢复。方法雄性C57BL/6小鼠经腹腔注射40 mg/kg·bw苯肼,于第0~12天内每2天鼠尾采血1次,检测外周血指标变化。在观察氢醌对溶血性贫血恢复的影响时,雄性C57BL/6小鼠第0天经腹腔注射苯肼40 mg/kg·bw,第4天经腹腔注射50 mg/kg·bw的氢醌,于第4天和第6天鼠尾采血进行检测。结果苯肼注射后12 d内,小鼠外周血RBC、HGB和PLT在第4天下降到最低水平,HCT在第2天下降到最低水平,MCV、MCH和RDW在第4天升高至最高水平,MCHC在第2天升高至最高水平,随后所有指标逐渐恢复至正常水平。单独注射氢醌不引起小鼠外周血相关指标明显变化;单纯苯肼注射组动物第6天的RBC、HGB和HCT相对于第4天出现明显的恢复性上升,而苯肼+氢醌组第6天的RBC、HGB和HCT明显低于苯肼组;苯肼组动物的MCHC和RDW在第4天显著高于对照组,第6天恢复性下降到对照水平,而苯肼+氢醌组第6天的MCHC和RDW明显高于苯肼组。结论苯肼单次注射可诱发小鼠轻度的溶血性贫血,注射氢醌可抑制贫血的恢复,表明苯肼诱发急性溶血性贫血动物模型可用于评价外源化合物的红系造血毒性。
Objective To observe whether hydroquinone can delay the recovery of phenylhydrazine-induced acute hemolytic anemia. Methods Male C57BL / 6 mice were injected intraperitoneally with phenylhydrazine (40 mg / kg · bw) and the blood samples were collected once every 2 days from the 0th to the 12th day to detect the change of peripheral blood indexes. To observe the effect of hydroquinone on the recovery of hemolytic anemia, male C57BL / 6 mice were injected intraperitoneally with phenylhydrazine 40 mg / kg · bw on day 0 and 50 mg / kg · bw hydroquinone by intraperitoneal injection on day 4, Rat tail blood was collected on day 4 and day 6 for testing. Results Within 12 days after phenylhydrazine injection, RBC, HGB and PLT in peripheral blood of mice decreased to the lowest level on the 4th day, HCT decreased to the lowest level on the 2nd day, MCV, MCH and RDW increased to the highest on the 4th day Level, MCHC increased to the highest level on the second day, then all indicators gradually returned to normal levels. Injection of hydroquinone alone did not cause significant changes in peripheral blood of mice; Phenylhydrazine injection group animals on the 6th day of RBC, HGB and HCT relative to the fourth day showed a clear recovery increased, and phenylhydrazine + hydroquinone group The RBC, HGB and HCT in 6 days were significantly lower than those in phenylhydrazine group. The MCHC and RDW in phenylhydrazine group were significantly higher than those in control group on day 4, and recovered to the control level on day 6, MCHC and RDW on day 6 were significantly higher than those on phenylhydrazine. Conclusions Single injection of phenylhydrazine can induce mild hemolytic anemia in mice, and injection of hydroquinone can restrain the recovery of anemia. It is indicated that the animal model of acute hemolytic anemia induced by phenylhydrazine can be used to evaluate the hematopoietic toxicity of exogenous compounds.