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目的:探讨前列腺上皮特异性Ets转录因子(prostate epithelium-specific Ets transcription factor,PDEF)在激素非依赖性前列腺癌(PCa)中的表达及其与Gleason分级和临床分期的相关性。方法:收集30例雄激素依赖性前列腺癌(A组)和30例雄激素非依赖性前列腺癌(B组)标本,运用免疫组织化学S-P法、Western blotting、RT-PCR半定量方法检测PDEF基因和蛋白的表达,并对照其临床分期和Gleason分级进行表达水平分析。结果:免疫组织化学实验显示,A组的PDEF阳性表达(70.0%,21/30)低于B组(96.7%,29/30,P<0.05)。Gleason分级≤7分组PDEF阳性表达率显著低于>7分组(64.3%vs 93.7%,P<0.01);临床分期>T2组PDEF阳性表达率显著高于≤T2组(86.8%vs 54.5%,P<0.01)。RT-PCR实验显示,A组与B组中PDEF mRNA的表达差异具有统计学意义(P<0.01),Gleason分级≤7分组PDEF mRNA表达率显著低于>7分组(P<0.01);临床分期>T2组PDEF mRNA表达显著高于≤T2组(P<0.01)。Western blotting进一步证实,PDEF蛋白表达与其mRNA表达变化相一致。结论:PDEF在雄激素非依赖性PCa标本中阳性表达率高,而且随临床分期和Gleason分级的升高其表达升高。提示PDEF参与了PCa的发生、发展及演变,可作为激素非依赖性PCa研究及治疗的靶向基因。
Objective: To investigate the expression of prostate epithelium-specific Ets transcription factor (PDEF) in hormone-independent prostate cancer (PCa) and its relationship with Gleason grade and clinical stage. Methods: Thirty male and female prostate cancers (group A) and 30 androgen-independent prostate cancer (group B) were collected. The expression of PDEF gene was detected by immunohistochemical SP method, Western blotting and semi-quantitative RT-PCR And protein expression, and compared its clinical stage and Gleason grade expression level analysis. Results: Immunohistochemistry showed that the positive expression of PDEF in group A (70.0%, 21/30) was lower than that in group B (96.7%, 29/30, P <0.05). The positive expression rate of PDEF in patients with Gleason grade ≤7 was significantly lower than that of> 7 (64.3% vs 93.7%, P <0.01). The positive expression rate of PDEF in patients with clinical stage> T2 was significantly higher than that in ≤T2 group (86.8% vs 54.5%, P <0.01). The expression of PDEF mRNA in group A and group B was significantly lower than that in group B (P <0.01), and the expression level of PDEF mRNA in group Gleason ≤7 was significantly lower than that in group B (P <0.01) > T2 group PDEF mRNA expression was significantly higher than the ≤ T2 group (P <0.01). Western blotting further confirmed that, PDEF protein expression and its mRNA expression consistent. Conclusion: The positive expression rate of PDEF in androgen-independent PCa specimens is high, and its expression is up-regulated with the increase of clinical stage and Gleason grade. PDEF is involved in the occurrence, development and evolution of PCa, which can be used as a target gene for hormone-independent PCa research and treatment.