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目的探究化学发光免疫法在甲胎蛋白患者中的检测效果及对结果的影响。方法使用化学免疫自动分析仪器对含有甲胎蛋白的血清进行检测,此过程中要开展测量范围验证与最大可允许稀释度两项实验,建立科学的可报告范围,服务于临床检测工作。结果测量范围为每毫升0.00 mg-912 mg,最大允许稀释度为1:50,实验测量值均值基本上符合分析仪灵敏度说明的数据。结论临床检验医师采用化学发光免疫法检测甲胎蛋白,可准确测定甲胎蛋白的测定范围及最大可允许的稀释度,为建立临床报告范围提供参考。
Objective To investigate the detection of chemiluminescence immunoassay in patients with alpha fetoprotein and its effect on the outcome. Methods The serum containing alpha-fetoprotein was detected by chemical immunoassay analyzer. Two steps of measuring range and maximum allowable dilution were carried out in this process, and a scientific reportable range was established to serve the clinical testing. Results The measurement range was 0.00 mg-912 mg / mL and the maximum allowable dilution was 1:50. The mean values of the experimental measurements were basically consistent with the analyzer sensitivity specifications. Conclusion The clinical laboratory physicians using chemiluminescence immunoassay detection of alpha-fetoprotein, alpha-fetoprotein can accurately determine the determination of the range and the maximum allowable dilution, to provide a reference for the establishment of clinical reporting range.