论文部分内容阅读
目的:观察针刀对帕金森病(PD)大鼠行为学及谷胱甘肽(GSH)含量的影响。方法:雄性SD大鼠随机分为空白组、模型组、电针组和针刀组(各16只),采用右侧纹状体双靶点注射6-羟多巴胺造模。电针组“百会”沿皮向双侧“太阳”透刺,每次20 min;针刀组取枕骨下项线中点和C1、C2横突后结节进行松解。进行行为学评价和黑质GSH含量检测。结果:空白组未见旋转行为,模型组干预治疗前后无差异(P>0.05);针刀大鼠旋转圈数明显减少且治疗前后相比有差异(P<0.01);电针组治疗前后相比有差异(P<0.05)。GSH含量比较,模型组GSH含量明显降低与空白组相比差异明显(P<0.01);针刀组和电针组GSH含量明显升高,针刀组、电针组与模型组相比有统计学差异(P<0.05)。结论:针刀可能通过改善PD旋转行为、提升GSH含量从而提高抗氧化能力减轻黑质DA能神经元损伤。
Objective: To observe the effect of acupotomy on behavior and glutathione (GSH) in Parkinson’s disease (PD) rats. Methods: Male Sprague-Dawley rats were randomly divided into blank group, model group, electro-acupuncture group and acupotome group (16 rats each). The right striatum was injected with 6-hydroxydopamine. Electroacupuncture group “Baihui ” along the skin to the bilateral “sun ” through the piercing, each 20 min; acupotome group under the occipital line and C1, C2 transverse nodules after loosening. Conduct behavioral evaluation and GSH content of substantia nigra. Results: There was no rotation in the blank group, and there was no difference in the model group before and after intervention (P> 0.05). The number of rotations of the acupotomized rats decreased significantly and the difference was significant (P <0.01) Than the difference (P <0.05). GSH content in model group was significantly lower than that in blank group (P <0.01); GSH content in acupotectomy group and electro-acupuncture group was significantly higher than that in model group Learning difference (P <0.05). Conclusion: The acupotomist may improve the anti-oxidation ability of DA and decrease the damage of DA neurons in substantia nigra by improving the rotation behavior of PD, increasing GSH content.