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12对杂种狗,每条体重13～22公斤。每次实验中一条为供心狗,制备离体心脏,另一条为供血的支持狗。供心狗在全麻、人工呼吸下开胸,左锁骨下动脉和右室分别插管后用交叉循环管连接于支持狗的颈总动脉和颈外静脉。然后将狗心切下,打开左房,切断左室腱索。将缚在球囊-泵连接器上的60ml(未扩展时)薄壁橡胶囊置于左室内,其开口与二尖瓣瓣环接牢。用微压力器测量左室压,等
12 pairs of mongrel dogs, each weighing 13 to 22 kg. In each experiment, one for the heart of the dog, preparation of isolated heart, and the other for the support of blood dogs. Congenital heart dogs in general anesthesia, artificial respiration thoracotomy, left subclavian artery and right ventricle after intubation with a cross-circulation tube connected to support the dog’s common carotid artery and external jugular vein. Then the dog heart cut, open the left atrium, cut off the left ventricular tendon cord. A 60 ml (non-expanded) thin walled rubber bladder tied to a balloon-pump connector was placed in the left ventricle and its opening was bolted to the mitral valve annulus. With a micro-pressure measurement of left ventricular pressure, and so on