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采用Ｂｏｒｎ氏比浊法观察了ｓｐｉｒａｍｉｎｅＮ－６在体外和体内对兔血小板聚集功能的影响．应用荧光分光光度法测定其对血小板５－ＨＴ释放反应的作用．结果：ｓｐｉｒａｍｉｎｅＮ－６在体外选择性抑制血小板活化因子（ＰＡＦ）诱导的血小板聚集，并呈量效关系，其ＩＣ５０＝２６μｍｏｌ／Ｌ；２ｍｇ／ｋｇｓｐｉｒａｍｉｎｅＮ－６静注后明显抑制ＰＡＦ和花生四烯酸（ＡＡ）诱导的血小板聚集，最大抑制率于给药后６０，３０ｍｉｎ分别为（５０４±４４）％和（７７３±３３）％（与生理盐水组对照Ｐ＜００５）；ｓｐｉｒａｍｉｎｅＮ－６呈浓度依赖性减少ＰＡＦ和ＡＡ引起血小板５－ＨＴ的释放，其ＩＣ５０分别为３３５ｕｍｏｌ／Ｌ和６４７μｍｏｌ／Ｌ．结果表明ｓｐｉｒａｍｉｎｅＮ－６是一种较强的抗血小板聚集剂．
Born’s turbidimetry was used to observe the effect of spiramine N-6 on rabbit platelet aggregation in vitro and in vivo. The fluorescence spectrophotometry was used to determine the effect of 5-HT on platelet release. RESULTS: Spiramine N-6 selectively inhibited platelet aggregation induced by platelet activating factor (PAF) in vitro and showed a dose-effect relationship. IC50 was 26 μmol/L; 2 mg/kg spiramine N-6 significantly inhibited PAF and arachidonic acid after intravenous injection. (AA) induced platelet aggregation, and the maximum inhibition rate was (504±44)% and (773±33)% at 60 and 30 min after administration (P<0 for saline group). 05);spiramineN-6 reduced the release of platelet 5-HT by PAF and AA in a concentration-dependent manner, with IC50 of 335umol/L and 647μmol/L, respectively. The results showed that spiramine N-6 is a strong anti-platelet aggregation agent.