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用转Bt单基因(FB56)和转Bt+CpTI双价基因(D18)杨树叶片饲喂杨小舟蛾Micromelalophatroglodyta(Graeser),采用酶活力测定方法研究了转基因杨树对幼虫中肠和血淋巴三种保护酶(SOD、CAT和POD)活力的影响。结果表明,幼虫中肠SOD活力显著升高,而CAT和POD活力受到了显著抑制。用FB56处理24h,杨小舟蛾幼虫中肠SOD活力增加了41.7%,与对照有显著差异;而用D18处理,SOD活力增加了25.3%,与对照差异不显著。用FB56处理24h,中肠CAT活力受到显著抑制,36h后抑制能力不强;而用D18处理,CAT活力全程受到显著抑制。用FB56和D18处理36h,中肠POD活力均受到显著抑制,分别比对照下降70.1%和89·8%。转基因杨树通过扰乱幼虫中肠SOD、CAT和POD三种保护酶的动态平衡,使虫体内自由基清除遇到了障碍,从而对其产生了毒害作用。转双价基因杨树对幼虫中肠保护酶的毒害作用大于转单基因杨树。用2种转基因杨树叶片饲喂杨小舟蛾幼虫后其血淋巴SOD和POD活力均没有受到显著影响,但CAT活力均显著高于对照,转基因杨树对血淋巴保护酶系统的影响小于对中肠保护酶系统的影响。
Microtlalophatroglodyta (Graeser) was fed on poplar leaves with transgenic Bt single gene (FB56) and transgenic Bt + CpTI gene (D18). The activity of transgenic poplar was evaluated in the midgut and hemolymph Effects of protective enzymes (SOD, CAT and POD) on vitality. The results showed that the larval midgut SOD activity was significantly increased, while CAT and POD activity was significantly inhibited. After treatment with FB56 for 24 h, SOD activity increased by 41.7% in the midgut of B. carnivore larvae, which was significantly different from the control. However, the SOD activity increased by 25.3% with D18 treatment, which was not significantly different from the control. After treatment with FB56 for 24 h, the CAT activity in the midgut was significantly inhibited and the inhibitory ability was not strong after 36 h. However, the CAT activity was significantly inhibited by D18 treatment. After treatment with FB56 and D18 for 36h, POD activity in the midgut was significantly inhibited by 70.1% and 89.8% respectively compared with the control. Transgenic Poplar through disturbing the midgut larvae of SOD, CAT and POD three kinds of protective enzymes in the dynamic balance of free radicals in the insect body encountered obstacles, thus causing its toxic effects. Transgenic poplar had more toxic effects on larval midgut protective enzymes than transgenic poplars. The activities of SOD and POD in hemolymph were not significantly affected by the feeding of two transgenic poplar leaves on larvae, but the CAT activity was significantly higher than that of the control. The effect of transgenic poplar on hemolymph protective enzyme system was less than that of control Intestinal protective enzyme system.