【摘 要】
：
蜡样芽胞杆菌(Bacillus cereus,B.cereus)是一种革兰阳性需氧芽孢杆菌,广泛分布于植物和土壤等环境中,它是一种食源性致病菌,通常引起食物中毒的B.cereus为呕吐型和腹泻型两
【机 构】
：
广西壮族自治区百色市乐业县疾病预防控制中心,
论文部分内容阅读
蜡样芽胞杆菌(Bacillus cereus,B.cereus)是一种革兰阳性需氧芽孢杆菌,广泛分布于植物和土壤等环境中,它是一种食源性致病菌,通常引起食物中毒的B.cereus为呕吐型和腹泻型两类,常在世界范围内引起食物中毒[1-3]。检测B.cereus的传统方法是培养法,但其敏感性低且耗时长。聚合酶链式反应(PCR),是一种DNA扩增技术,当存在模板DNA、底物、上下游引物和耐热DNA聚合酶时,经过多次“变性-复性-延伸反
Bacillus cereus (Bacillus cereus, B.cereus) is a Gram-positive aerobic Bacillus, widely distributed in plants and soil environment, it is a foodborne pathogenic bacteria, usually caused by food poisoning B .cereus vomiting and diarrhea type, often causing food poisoning in the world [1-3]. The traditional method of detecting B.cereus is culture, but its sensitivity is low and it takes a long time. Polymerase chain reaction (PCR) is a DNA amplification technique that, when present in template DNA, substrate, upstream and downstream primers, and thermostable DNA polymerase, undergoes multiple ”denaturation-renaturation-extension
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