【摘 要】
：
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever disease caused by SFTSV,a newly discovered phlebovirus that is named after the disease.Currently,no effective vaccines or drugs are available for use against SFTSV infecti
【机 构】
：
School of Life Sciences,Tianjin University,Tianjin 300073,China;School of Environmental Science and
论文部分内容阅读
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever disease caused by SFTSV,a newly discovered phlebovirus that is named after the disease.Currently,no effective vaccines or drugs are available for use against SFTSV infection,as our understanding of the viral pathogenesis is limited.Bortezomib (PS-341),a dipeptideboronic acid analog,is the first clinically approved proteasome inhibitor for use in humans.In this study,the antiviral efficacy of PS-341 against SFTSV infection was tested in human embryonic kidney HEK293T (293T) cells.We employed four different assays to analyze the antiviral ability of PS-341 and determined that PS-341 inhibited the proliferation of SFTSV in 293T cells under various treatment conditions.Although PS-341 did not affect the virus absorption,PS-341 treatment within a non-toxic concentration range resulted in a significant reduction of progeny viral titers in infected cells.Dual-luciferase reporter assays and Western blot analysis revealed that PS-341 could reverse the SFTSV-encoded non-structural protein (NS) mediated degradation of retinoic acid-inducible gene-1 (RIG-I),thereby antagonizing the inhibitory effect of NSs on interferons and blocking virus replication.In addition,we observed that inhibition of apoptosis promotes virus replication.These results indicate that targeting of cellular interferon pathways and apoptosis during acute infection might serve as the bases of future therapeutics for the treatment of SFTSV infections.
其他文献
Dear Editor,rnIncreasing number of hepatitis E virus (HEV) infection has been described in industrialized countries,with infection occurring as a sporadic disease,usually associated with travel to endemic regions or small outbreaks due to ingestion of con
Dear Editor,Parainfluenza virus 5 (PIV5),known as canine parain-fluenza virus in the veterinary field,is a negative-sense,nonsegmented,single-stranded RNA virus belonging to the Paramyxoviridae family (Chen 2018).The virus was first reported in primary mo
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans.Although dromedary camels are considered as a major reservoir host,the MERS-CoV infection dynamics in camels are not ful
As we know more about Zika virus (ZIKV),as well as its linkage to birth defects (microcephaly) and autoimmune neurological syndromes,we realize the importance of developing an efficient vaccine against it.Zika virus disease has affected many countries and
Dear Editor,The fall armyworm,Spodoptera frugiperda (Lepidoptera:Noctuidae),a highly agricultural destructive pest of cereals,is originally rampant in tropical and subtropical America (Kenis et al.2019).The caterpillars of this moth feed on more than 350
RNA granules are cytoplasmic,microscopically visible,non-membrane ribo-nucleoprotein structures and are important posttranscriptional regulators in gene expression by controlling RNA translation and stability.TIA/G3BP/PABP-specific stress granules (SG) an
Cellular microRNAs (miRNAs) have been shown to modulate HCV infection via directly acting on the viral genome or indirectly through targeting the virus-associated host factors.Recently we generated a comprehensive map of HCV-miRNA interactions through gen
During human immunodeficiency virus (HIV) infection,type Ⅰ interferon (IFN-Ⅰ) signaling induces an antiviral state that includes the production of restriction factors that inhibit virus replication,thereby limiting the infection.As seen in other viral inf
Enteric viruses are the most common cause of acute gastroenteritis (AGE) in young children and a significant public health problem globally.Hospital admissions of children under 5 years of age with diarrhea are primarily associated with group A rotavirus
Dear Editors,rnThe coronavirus disease 2019 (COVID-19), caused by SARS-CoV-2, broke out in early December 2019 has escalated into a global pandemic (Lai et al.2020).Till the May 20th 2020, more than 4,700,000 people were infected and the number is still i