论文部分内容阅读
目的:建立毛细管区带电泳同时测定不同产地三棵针中盐酸小檗碱、盐酸药根碱、盐酸巴马汀含量的方法。方法:空心石英毛细管(75 cm×50μm)柱,以50 mmol.L-1硼砂-甲醇(2∶1)的溶液为电泳介质,运行电压25 kV,柱温15℃,检测波长230 nm。结果:盐酸小檗碱、盐酸药根碱及盐酸巴马汀分别在0.816 8～32.672 0 mg.L-1(r2=0.999 4),0.852 0～8.520 0 mg.L-1(r2=0.999 5),0.815 2～8.152 0 mg.L-1(r2=0.999 6)线性关系良好;其平均加样回收率分别为103.8%(RSD 1.14%),100.7%(RSD 3.46%),100.2%(RSD 2.87%)。结论:实验方法快速简便,准确可靠,适用于三棵针等药材中盐酸小檗碱、盐酸巴马汀及盐酸药根碱的含量测定。
OBJECTIVE: To establish a method for simultaneous determination of berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride in Sanqiao acupoints of different producing areas by capillary zone electrophoresis. METHODS: Hollow silica capillary column (75 cm × 50 μm) was used as the electrophoresis medium with a solution of 50 mmol·L-1 borax-methanol (2:1) at a voltage of 25 kV with a column temperature of 15 ° C and a detection wavelength of 230 nm. Results: Berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride were respectively 0.816 8 ~ 32.672 0 mg.L-1 (r2 = 0.999 4), 0.852 0 ~ 8.520 0 mg.L-1 (r2 = 0.999 5 ), 0.815 2 ~ 8.152 0 mg.L-1 (r2 = 0.999 6). The average recoveries were 103.8% (RSD 1.14%), 100.7% (RSD 3.46%) and 100.2% 2.87%). Conclusion: The experimental method is rapid, simple, accurate and reliable, and is suitable for the determination of berberine hydrochloride, palmatine hydrochloride and jatrorrhizine hydrochloride in three herbs.