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目的以巴戟天药材生品为基础,建立巴戟天不同炮制品的高效薄层色谱(HPTLC)指纹图谱,并比较分析生品的指纹图谱。方法硅胶GF254高效预制薄层板,乙酸乙酯部位以石油醚-乙酸乙酯(5∶5.5)为展开剂,上行展开8 cm,10%硫酸乙醇溶液显色;正丁醇部位以正丁醇-冰醋酸-水(4∶1∶5)上层溶液为展开剂,上行展开8 cm,α-萘酚浓硫酸试剂显色。显色后置紫外光灯365 nm或自然光下检视,得薄层色谱指纹图谱,并进行相关分析。结果巴戟天生品的薄层荧光色谱指纹图谱与巴戟肉(蒸制)、盐巴戟天、制巴戟天(甘草制)图谱比较分析,彼此具有明显的区别,可用于生品与巴戟天炮制品的鉴别。结论通过HPTLC指纹图谱考察,生品巴戟天与巴戟肉、盐巴戟天、制巴戟天的区别较大,应分别制定质量评价的标准。
OBJECTIVE To establish HPLC fingerprints of different products from Morinda officinalis and compare the fingerprints of the crude products. Methods Silica gel GF254 was prefabricated in a thin layer. The ethyl acetate fraction was extracted with petroleum ether - ethyl acetate (5: 5.5) - glacial acetic acid - water (4: 1: 5) upper solution as developing solvent, unfolded 8 cm upward, α-naphthol concentrated sulfuric acid reagent color. Post-color UV light 365 nm or natural light view, the thin-layer chromatography fingerprints and correlation analysis. Results The TLC fingerprints of Morinda officinalis crude products were compared with those of the morinda officinalis (Steamed), Morinda officinalis (Morinda officinalis), and Morinda officinalis (Licorice) Identification of the days of products. Conclusion According to HPTLC fingerprinting, the difference between Morinda officinalis and Morinda officinalis, Morinda officinalis, and Morinda officinalis is larger. Quality standards should be established separately.