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在含２，４－Ｄ２ｍｇ／１的Ｃ１７基本培养基中附加浓度为０．１，０．３，０．５，０．７和０．９ｍｇ／１的氯苯胍，用于培养烟农×８４－Ａ－３５９－９、新冬１５×８４－Ａ－３５９－９、唐山６８９８×８８－Ａ－９１６－１、花冬８６－１６×太原８１、８８－１３８×８４－Ａ－３５９－９和花冬８６－１６×２７－Ａ－４５５－１小麦杂交组合Ｆ２代花药，结果花粉愈伤组织平均诱导率为３．２１％－４．７９％，其中０．７（４．３２％）和０．９（４．７９％）的诱导率高于对照（４．０９％），其他均低于对照；绿苗分化率（１１．９７％－１９．４０％）均低于对照（２０．１９％），但以０．９ｍｇ／ｌ（１９．４％）与对照接近；白苗分化率（１０．１６％－１５．４９％）均高于对照（６．７３％）。
In the C17 minimal medium containing 2,4-D2 mg / l, chlorpromazine at concentrations of 0.1, 0.3, 0.5, 0.7 and 0.9 mg / l was added to cultivate tobacco farmers × 84 -A-359-9, New Winter 15 × 84-A-359-9, Tangshan 6898 × 88-A-916-1, Huadong 86-16 × Taiyuan 81, 88-138 × 84-A-359-9 And Huadong 86-16 × 27-A-455-1. The average pollen callus induction rate was 3.21% -4.79%, of which 0.7 (4.32%) And 0.9 (4.79%) were higher than that of the control (4.09%), and the others were lower than the control. The differentiation rate of green plantlets (11.97% -19.40%) was lower than that of the control .19%), but 0.9 mg / l (19.4%) was close to the control. The rate of white seedlings was higher than that of the control (10.16% -15.49%).