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目的 观察氧化苦参碱体外抗乙型肝炎病毒 (HBV)的活性。方法 以HepG2 2 .2 .15细胞株为模型 ,用微粒酶免疫测定技术 (MEIA)检测细胞培养上清液中HBsAg、HBeAg含量 ,用bDNA信号扩增法定量检测细胞内核心颗粒HBVDNA的变化 ,以MTT比色法观察药物的细胞毒性。结果 氧化苦参碱 2 0 0 0 μg/ml时对HBsAg、HBeAg的抑制率分别达 40 .5 7%、48.2 7% ;浓度为 10 0～ 2 0 0 0 μg/ml时 ,能明显降低 2 .2 .15细胞胞浆核心颗粒HBVDNA水平 ;无明显细胞毒性作用。结论 体外细胞培养表明 ,氧化苦参碱具有直接抗HBV活性
Objective To observe the activity of oxymatrine against hepatitis B virus (HBV) in vitro. Methods The HepG2 2 .2.15 cell line was used as a model to detect the HBsAg and HBeAg levels in the cell culture supernatant by MEIA. The changes of HBVDNA in the nucleus were detected by bDNA signal amplification. The cytotoxicity of the drug was observed by MTT colorimetry. Results The inhibition rates of HBsAg and HBeAg were 40.57% and 48.27% respectively when the oxymatrine was at 2000 μg / ml, and the inhibitory rates of HBsAg and HBeAg at the concentration of 10 0 ~ 20 000 μg / ml were significantly decreased by 2 .2 .15 cells cytoplasmic core particles HBVDNA levels; no significant cytotoxicity. Conclusion In vitro cell culture shows that oxymatrine has a direct anti-HBV activity