目的探讨重组腺病毒载体介导的血管形成素1(Ang1)基因转染对兔急性心梗后左室功能影响。方法构建含有Ang1的腺病毒粘粒载体,通过转染293细胞进行同源重组制备重组腺病毒颗粒。64只雄性新西兰大白兔行高位冠状动脉结扎,4只用于急性心梗后循环中VEGF含量的检测,其余60只随机均分为实验组、单纯培养基组(DMEM)与LacZ重组腺病毒组,分别于冠状动脉结扎后心肌内直接注射Ang1重组腺病毒,DMEM与LacZ重组腺病毒。并于转染后第3、14、28d应用超声心动图观察心功能情况,第3、7、14、28d应用RT PCR方法测定重组腺病毒基因在转染心肌中的表达,第14、28d用免疫组化方法观察缺血心肌内血管生长情况。结果获得的Ang1重组腺病毒滴度为5.6×1011pfu/L。重组腺病毒直接注射转染兔缺血心肌后能有效表达目的基因,RT PCR测定提示Ang1基因转染后第3d心肌中即有表达,7、14d仍呈阳性表达,28d未测出。心肌梗死后Ang1组心功能从术后第3d至第28d得到明显改善,其幅度显著好于对照组。心肌梗死后左室心肌收缩期截面积与舒张期截面积均明显增加,尤以术后第3d为著,随时间延长均有所缩小,至28d后Ang1组缩小幅度明显高于其他两组。转染14、28d后Ang1组新生毛细血管密度及αSMA阳性的小动脉性血管密度均明显高于对照组(P<0.01)。结论腺病毒介导的Ang1基因在兔缺血心肌中能有效地促进新生血管形成,并能改善缺血心肌的功能。 Objective To investigate the effect of recombinant adenovirus vector-mediated angiopoietin 1 (Ang1) gene transfection on left ventricular function after acute myocardial infarction in rabbits. Methods The adenovirus cosmid vector containing Ang1 was constructed, and the recombinant adenovirus particles were prepared by homologous recombination by transfecting 293 cells. Sixty-four male New Zealand white rabbits underwent coronary artery ligation. Four of them were used to detect the content of VEGF in the circulation after acute myocardial infarction. The other 60 were randomly divided into experimental group, DMEM group and LacZ recombinant adenovirus group , Were injected directly after coronary artery ligation of Ang1 recombinant adenovirus, DMEM and LacZ recombinant adenovirus. The cardiac function was observed by echocardiography on the 3rd, 14th and 28th day after transfection. The expression of recombinant adenovirus gene in transfected myocardium was detected by RT-PCR at the 3rd, 7th, 14th, 28th day. Immunohistochemistry was used to observe the vascular growth in ischemic myocardium. Results The titer of Ang1 recombinant adenovirus was 5.6 × 1011pfu / L. The recombinant adenovirus directly transfected rabbit ischemic myocardium can effectively express the target gene, RT PCR assay Ang1 gene transfection that is expressed in the third 3d myocardial, 7,14 d still positive expression, 28 d did not detect. After myocardial infarction Ang1 heart function improved significantly from 3d to 28d after surgery, the amplitude was significantly better than the control group. After myocardial infarction, left ventricular myocardial systolic cross-sectional area and diastolic cross-sectional area were significantly increased, especially in the third postoperative as time increases with time have been reduced to 28d Ang1 group was significantly smaller than the other two groups. The density of newborn capillaries and the arterial density of α SMA positive in Ang1 group were significantly higher than those in control group (P <0.01) 14 and 28 days after transfection. Conclusion Adenovirus-mediated Ang1 gene can effectively promote neovascularization in ischemic myocardium and improve the function of ischemic myocardium.