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目的 :用Boyden小室侵袭试验测定膀胱癌细胞的体外侵袭力 ,并探索肿瘤细胞侵袭与其它生物学习性之间的关系。方法 :采用人工基底膜构建侵袭小室。测定EJ、T2 4、BIU 873个膀胱癌细胞株的体外侵袭力 ,并测定细胞的生长曲线、集落形成率、体外趋化运动性等指标 ,分析这些指标与体外侵袭力的关系。用扫描电镜观察侵袭过程中癌细胞的形态变化。结果 :EJ、T2 4、BIU - 87的侵袭细胞数分别为 :39 2 6± 13.90、77 0 0± 13 2 8、166 53± 2 7 2 0 ,癌细胞的体外侵袭力分别与细胞的体外运动性和生长曲线有密切关系。电镜下可见细胞通过变形运动穿过微孔 ,并伸出丝状伪足来协助运动。结论 :Boyden小室侵袭试验为测定体外癌细胞侵袭力较可靠的方法。肿瘤的侵袭力与细胞的某些生物学习性有关 ,因而测定有关指标也可间接判断肿瘤的侵袭力。
OBJECTIVE: To determine the invasiveness of bladder cancer cells in vitro using Boyden chamber invasion assay and to explore the relationship between tumor cell invasion and other biological behaviors. Methods: Artificial basement membrane was used to construct invasion chamber. The in vitro invasiveness of bladder cancer cell lines EJ, T2 4 and BIU were determined. The growth curve, colony formation rate, chemotactic mobility and other indexes were determined, and the relationship between these indexes and invasiveness was analyzed. Scanning electron microscopy was used to observe the morphological changes of cancer cells during invasion. Results: The invasive cell numbers of EJ, T2 4 and BIU - 87 were 39 2 6 ± 13.90 and 77 0 0 ± 132 8,166 53 ± 2 7 2 0, respectively. In vitro invasiveness of cancer cells was correlated with the cell in vitro Exercise and growth curve are closely related. Electron microscopy shows cells through the deformation of the movement through the micropores, and extended filopodia to assist the movement. Conclusion: Boyden chamber invasion assay is a more reliable method to determine invasiveness of cancer cells in vitro. The invasiveness of the tumor cells and some biological habits related to determination of the relevant indicators can also indirectly determine the invasiveness of the tumor.