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目的 用1 8F 脱氧葡萄糖 (FDG)、99Tcm 甲氧基异丁基异腈 (MIBI)评价犬冷冻心肌骨髓CD34+ 细胞移植后心肌代谢和灌注的变化。方法 1 2只杂种犬分为细胞移植组和对照组。用免疫磁珠法从犬肋骨骨髓分离CD34+ 细胞并注射到用CO2 冷冻建立的慢性心肌梗死模型区 ,对照组注射伊思考夫改良杜尔贝可培养基 (IMDM)培养液。分别于建立模型前、后 4周和干细胞移植后 8周行1 8F FDG和99Tcm MIBI心肌显像 ,评价细胞移植结果 ,计算冷冻心肌代谢与灌注显像的F值。干细胞移植后 8周取心肌做第 8因子免疫组织化学和病理检查。结果 正常心肌代谢与灌注显像清晰 ,F值接近 0 ,干细胞移植前、后 8周冷冻心肌1 8F FDG与99Tcm MIBI显像的F值分别为 5 . 5 0± 1 . 31 ,5 4 4± 0 . 70与 1 1 7±0. 4 1 ,1 . 5 0± 0 . 5 5 (P <0 . 0 1 ) ;对照组为 5 . 5 3± 0. 80 ,5. 5 4± 1 . 2 9与 5. 0 0± 1 . 5 5 ,5 . 0 8± 1. 4 6 (P >0 . 0 5 ) ,骨髓CD34+ 细胞移植使冷冻心肌的代谢与灌注明显得到恢复。干细胞移植后 8周冷冻区血管密度高于对照组 (P <0 . 0 1 )。结论 骨髓CD34+ 细胞移植后冷冻区存在大量活的心肌细胞。
Objective To evaluate the changes of myocardial metabolism and perfusion after transplantation of canine frozen myocardium CD34 + cells by using 18F deoxyglucose (FDG) and 99Tcm methoxyisobutylisonitrile (MIBI). Methods 1 2 hybrid dogs were divided into cell transplantation group and control group. CD34 + cells were isolated from canine rib marrow by immunomagnetic beads method and injected into the model of chronic myocardial infarction established by CO2 freezing. The control group was injected with Ikkofu modified Dulbecco’s modified medium (IMDM). The myocardial imaging of 18 F FDG and 99 Tcm MIBI were performed 4 weeks before and 4 weeks after the establishment of the model and 8 weeks after the stem cell transplantation respectively to evaluate the cell transplantation results and calculate the F value of myocardial metabolism and perfusion imaging. Cardiomyocytes were harvested at 8 weeks after stem cell transplantation for factor 8 immunohistochemistry and pathology. Results Normal myocardial metabolism and perfusion imaging were clear and the F value was close to 0. The F values of 18F FDG and 99Tcm MIBI images of frozen myocardium before and 8 weeks after stem cell transplantation were respectively 5.50 ± 1.31 and 544 ± 0. 70 and 1 1 7 ± 0 4 1, 1.5 0 ± 0.55 (P <0. 0 1). The control group was 53.3 ± 0.80 and 5.54 ± 1. The bone marrow CD34 + cell transplantation significantly restored the metabolism and perfusion of frozen myocardium. The density of blood vessels in the frozen zone at 8 weeks after stem cell transplantation was higher than that in the control group (P <0.01). Conclusion There are a large number of live cardiomyocytes in frozen zone after transplantation of bone marrow CD34 + cells.