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选用晋旱125×(昔野×501)杂交组合的亲本及后代所产生的有代表性的四个特殊类型73-1(尖叶白花)、73-2(圆叶紫花)、73-3(圆叶白花)、73-4(尖叶紫花)为试验材料,分别在正常供水和干旱胁迫的条件下,测定苗期和花荚期的POD、SOD、CAT等与抗旱性相关的保护酶指标,并对株高、株荚数、百粒重等农艺性状进行综合分析,还对亲本和子代的抗旱性差异情况用抗旱性隶属函数值(D值)进行了综合的测评,结果表明:干旱胁迫下,不同品系的保护酶活性均有所变化,且在不同时期,各种酶的变化程度不同;在大豆对水分最为敏感的花荚期对SOD、POD、CAT的活性进行测定和分析,采用抗旱隶属函数值的方法综合测评,认为这一群体中抗旱性从强到弱的次序为:晋旱125、73-2(圆叶紫花)、昔野×501、73-1(尖叶白花)、73-3(圆叶白花)、73-4(尖叶紫花)。
Three representative types of 73-1 (Spike white flower), 73-2 (Round flower), 73-3 (Pseudostellaria lobata), 73-4 (Ziziphus chinensis) were used as materials to determine the protective enzyme indexes related to drought resistance, such as POD, SOD and CAT at the seedling and flowering stage under the condition of normal water supply and drought stress respectively. , And analyzed the agronomic traits such as plant height, pod number and 100-grain weight. The differences of drought resistance between parents and offspring were also evaluated by drought resistance membership value (D value). The results showed that drought Under the stress, the activities of protective enzymes in different lines all changed, and the degrees of changes of different enzymes were different at different stages. The activities of SOD, POD and CAT were determined and analyzed in the flower-stage of soybean that was most sensitive to water, Based on the comprehensive evaluation of the membership function value of drought resistance, the order of drought resistance in this group was as follows: Jinhan 125,73-2 (Round leaf violet), Xingye × 501,73-1 ), 73-3 (round white flowers), 73-4 (sharp purple flowers).