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人体感染乙型肝炎病毒(HBV)后血清中病毒标志物(HBVM),由于病毒感染状态及机体免疫应答水平之差异,而在血清中的表达有明显不同。检测 HBVM对于 HBV 致病性及免疫性的研究,对于 HBV 感染的诊断、疗效观察及预后判断有重要意义。近年来,由于聚合酶链反应(Poly-merase Chain Reaction,PCR)检测技术的开展,使乙型肝炎研究在分子水平上跨越了一大步。PCR 检测 HBV-DNA 的敏感水平最高可达10~(-6)Pg,而过去公认敏感的斑点杂交法的敏感水平仅
The serum HBV markers (HBVMs) in human beings infected with Hepatitis B virus (HBV) are significantly different in serum due to differences in viral infection status and immune response. Detection of HBVM for pathogenicity and immunity of HBV research, for the diagnosis of HBV infection, the efficacy of observation and prognosis of great significance. In recent years, the study of hepatitis B has made a big leap forward on molecular level due to the development of Poly-merase Chain Reaction (PCR) detection technology. PCR detection of HBV-DNA sensitivity up to 10 ~ (-6) Pg, while in the past the sensitivity of sensitive dot blot hybridization only sensitive level