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目的:比较重症肌无力(MG)患者与非自身免疫对照组胸腺组织CMTM8、CKLF1、相关趋化因子及受体的表达,为揭示MG胸腺异常及其发生机制提供理论依据。方法:用基因芯片筛选差异表达的趋化因子基因,采用RT-PCR和实时荧光定量PCR方法分别检测正常胸腺和MG胸腺中CMTM8 mRNA的表达,用免疫组织化学方法对CKLF1蛋白的表达和分布进行检测。结果:增生型MG胸腺组织中CMTM8 mRNA的表达明显高于正常人胸腺(2-△△Ct=8.3),差异有统计学意义;CKLF1蛋白在正常胸腺、MG胸腺中的均有广泛的表达,阳性细胞主要为胸腺基质细胞;CXCR6、CCL2、CCL8、CX3CL1、CXCL10和CCR1在MG患者胸腺表达水平显著低于对照组;CCL22、CCL3、CXCL12、CCR5、CCR7和CCR9基因在MG患者胸腺表达水平显著高于对照组。结论:CMTM8及多种趋化因子基因在增生型MG胸腺的表达明显高于对照组胸腺,可能在MG患者T细胞异常发育的某些阶段起重要作用。
OBJECTIVE: To compare the expressions of CMTM8, CKLF1, chemokines and their receptors in thymus of patients with myasthenia gravis (MG) and non-autoimmune controls, and to provide a theoretical basis for revealing the abnormalities of thymus and their pathogenesis. Methods: The differentially expressed chemokine genes were screened by gene chip. The expression of CMTM8 mRNA in normal thymus and MG thymus was detected by RT-PCR and real-time fluorescence quantitative PCR respectively. The expression and distribution of CKLF1 protein were detected by immunohistochemistry Detection. Results: The expression of CMTM8 mRNA in proliferative MG thymus was significantly higher than that in normal human (2 - △△ Ct = 8.3), the difference was statistically significant. CKLF1 protein was widely expressed in normal thymus and MG thymus, The expression of CCL22, CCL3, CXCL12, CCR5, CCR7 and CCR9 genes in MG patients was significantly lower than that in control group Higher than the control group. CONCLUSION: The expression of CMTM8 and various chemokines in the thymus of proliferative MG was significantly higher than that of the control group, which may play an important role in some stages of abnormal T cell development in MG patients.