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本文通过高效液相色谱技术(HPLC)测定紫杉醇含量并与其药材的傅里叶红外光谱(FTIR)进行相关分析,研究不同肥料处理下曼地亚红豆杉药材紫杉醇含量相关的特征峰,并比较分析不同浓度复合肥对曼地亚红豆杉紫杉醇含量的影响。结果显示:不同处理间红外吸收光谱的峰形和峰位置均比较接近,但各谱峰的峰强有一定差异;为进一步研究与紫杉醇含量相关的吸收峰,对3369、1732、1616、1445、1373、1245、1147、1104、1056 cm-1等处的峰强与实测紫杉醇含量的关系进行多元对数相关回归分析,结果显示:在1445、1373、1317、1245、1147、1104、1056 cm-1等处附近的吸收强度与实际测定紫杉醇含量均密切相关,R(复相关系数)=0.98,P(概率)=0.002;两种方法均一致显示,在春梢抽梢前施少量的复合肥对紫杉醇含量积累有一定促进作用,但复合肥>20 g/株时反而不利于紫杉醇的积累;四个物候期比较,以春梢抽梢前期施用复合肥对紫杉醇的累积影响最大。本研究结果对曼地亚红豆杉良种选育和规范化栽培技术研究均具有重要的指导意义。
In this paper, the content of paclitaxel was determined by high performance liquid chromatography (HPLC) and correlated with the FTIR spectra of its medicinal materials. The characteristic peaks of paclitaxel content of Taxus mairei under different fertilizer treatments were compared and analyzed Effect of different concentrations of compound fertilizer on paclitaxel content in Taxus media. The results showed that the peak shape and peak position of infrared absorption spectra of different treatments were close, but the peak intensity of each peak was different. To further study the absorption peaks related to the content of paclitaxel, the peaks of 3369, 1732, 1616, 1445, 1373,1245,1147,1104,1056 cm-1 peak intensity and the measured content of paclitaxel multivariate logistic regression analysis showed that: at 1445,1373,1317,1245,1147,1104,1056 cm- 1 and so on were closely related to the actual determination of paclitaxel content. R (complex correlation coefficient) = 0.98, P (probability) = 0.002. Both methods showed that the application of a small amount of compound fertilizer However, the compound fertilizer> 20 g / plant was not conducive to the accumulation of paclitaxel. Compared with the four phenophases, the compound fertilizer had the greatest impact on the accumulation of paclitaxel in the early shoots. The results of this study on the Taxus mairei elite breeding and standardization of cultivation techniques have an important guiding significance.