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目的:探讨影响抗原特异性Th17细胞分化和调节的因素。方法:T细胞受体转基因小鼠(DO11.10)CD4+T细胞,与同背景正常小鼠的脾细胞混合,经OVA多肽刺激后,在不同的Th17极化的培养条件下,观察Th17细胞及细胞因子的产生。结果:单纯OVA抗原刺激主要诱导特异性Th1型反应,在TGF-β和IL-6存在的条件下,主要诱导Th17反应;当加入IL-23之后,促进了Th17细胞的分化。阻断了IFN-γ和IL-4之后,Th17细胞的百分率明显增加。LPS可以促进Th1型细胞因子的产生,但对抗原特异性Th17细胞的分化没有明显的促进作用。结论:抗原特异性Th17细胞是与Th1、Th2相对独立的细胞亚群,TGF-β、IL-6和IL-23可诱导或促进Th17的分化,而Th1和Th2细胞因子抑制其分化。
Objective: To explore the factors affecting the differentiation and regulation of antigen-specific Th17 cells. Methods: CD4 + T cells from T cell receptor transgenic mice (DO11.10) were mixed with splenocytes from normal mice. After stimulation with OVA peptide, Th17 cells were observed under different conditions of Th17 polarization And cytokine production. Results: The OVA antigen stimulation induced the specific Th1-type response and induced the Th17 response mainly in the presence of TGF-β and IL-6. The differentiation of Th17 cells was promoted when IL-23 was added. After blocking IFN-γ and IL-4, the percentage of Th17 cells was significantly increased. LPS can promote the production of Th1-type cytokines, but did not promote the differentiation of antigen-specific Th17 cells. Conclusion: The antigen-specific Th17 cells are relatively independent of Th1 and Th2 cell subsets. TGF-β, IL-6 and IL-23 can induce or promote the differentiation of Th17 cells. Th1 and Th2 cytokines can inhibit the differentiation of Th17 cells.