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为研究转Ch IFN-α基因百脉根的低温生长特性,对其进行4℃低温离体水培和可溶性蛋白(SP)、丙二醛(MDA)、超氧化物歧化酶(SOD)和脯氨酸(Pro)的测定分析,探讨低温胁迫对转Ch IFN-α基因百脉根的影响及机制。研究发现,25 d低温处理期间,百脉根植株均生长迟缓,野生型(WT)还出现叶片枯黄和茎基部腐烂等冻害现象。转基因百脉根(TP)植株同期的MDA含量比WT低1.03倍(p<0.01),WT的MDA下降幅度仅为TP植株的86%。TP同期的SP、SOD、Pro含量均较WT高(p<0.01),且出现的降幅小。TP同期的SP、SOD和Pro含量分别是WT的1.21倍、1.27倍和1.72倍(p<0.01),WT同期的SP和SOD降幅分别是TP的2.26倍和1.93倍(p<0.01),TP植株的SOD含量峰值比WT提前5 d,TP的Pro在中后期增至WT植株的150%和129%。结果表明,转基因百脉根具有较好的低温耐受性和耐受生理基础,这对其在高寒山区的进一步应用及管理提供了基础依据。
In order to study the hypothermic growth characteristics of ChIFN-α transgenic Lotus japonicus, the in vitro culture in vitro at 4 ℃ and the content of soluble protein (SP), malondialdehyde (MDA), superoxide dismutase (SOD) (Pro) assay to investigate the effects of chilling stress on the transcript of ChIFN-α gene Lotus root and its mechanism. The results showed that the growth of L. officinalis plants was slow during the 25 days of low temperature treatment, and the frost damage of the leaves of the wild type (WT) was also observed. The MDA content of transgenic plants was 1.03 times lower than that of WT (p <0.01). The MDA content of WT decreased only 86% of that of TP plants. TP at the same period of SP, SOD, Pro levels were higher than WT (p <0.01), and the decline was small. The levels of SP, SOD and Pro at the same period of TP were 1.21-fold, 1.27-fold and 1.72-fold higher than those of WT, respectively (p <0.01) The SOD peak of the plant was 5 days earlier than that of the WT, and the Pro of TP increased to 150% and 129% of the WT plants in the middle and late stages. The results showed that transgenic Lotus japonicus had good low temperature tolerance and physiological basis, which provided the basis for its further application and management in the alpine region.