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目的 :了解丙型肝炎病毒 (HCV)感染的基因型及其与临床的关系。方法 :用逆转录套式聚合酶链反应 (RT PCR)和限制性片断长度多态性 (RFLP)分析的方法 ,对 78例HCV感染患者进行HCVNS5区基因分型。结果 :HCV 1b型感染 71例 (91% ) ,HCV 2a型 7例 (9% ) ,未发现其它基因型 ;丙型肝炎患者有无输血史、是否重叠HBV感染 ,其基因分型构成比均无显著性差异 (P >0 .0 5 ) ;1b型HCV血清ALT异常者 (6 3.4% )明显多于 2a型(14.3 % ) (P <0 .0 5 )。结论 :本组HCV感染的基因型多数为 1b型 ,少数为 2a型 ,1b型更易致肝细胞损伤 ,并可能与活动性肝病有关
Objective: To understand the genotype of hepatitis C virus (HCV) infection and its relationship with clinical. Methods: HCV NS5 genotypes were genotyped in 78 patients with HCV infection by reverse transcription polymerase chain reaction (RT PCR) and restriction fragment length polymorphism (RFLP). Results: HCV genotype 1b infection was found in 71 cases (91%) and HCV 2a genotype in 7 cases (9%). No other genotypes were found in HCV genotypes. There was no significant difference between the two groups (P> 0.05). The abnormal serum ALT level of type 1b HCV genotype was significantly higher than that of type 2a genotype (3.4%) (P <0.05). Conclusion: The majority of genotypes of HCV infection in this group are 1b type, a small number of 2a type, 1b type more likely to cause liver cell damage, and may be related to active liver disease