论文部分内容阅读
目的探讨环氧合酶-1(COX-1)、环氧合酶-2(COX-2)基因多态性与阿司匹林抵抗相关性。方法将96例缺血性脑卒中患者分为阿司匹林敏感组87例和阿司匹林非敏感组9例。2组患者口服100 mg阿司匹林至少一周。用光比浊法对患者的血小板聚集度进行测定,用Sequenom Mass A RRAY i PLEX基因型分析技术进行COX-1、COX-2的基因型检测,分析COX-1、COX-2基因多态性与阿司匹林的相关性。结果阿司匹林非敏感组患者的既往卒中史比例66.7%(6/9)显著高于阿司匹林敏感组21.9%(19/87)(P<0.05)。2组患者的COX-1(rs1330344,rs3842788)基因型分布差异有统计学意义(P<0.05),但COX-1(rs5788)、COX-2(rs20417)基因型分布差异无统计学意义(P>0.05)。COX-1(rs5788,rs1330344,rs3842788)、COX-2(rs20417)等位基因及基因型频率4个单核苷酸基因多态性均符合Hardy-Weinberg平衡(P>0.05)。结论 COX-1(rs3842788,rs1330344)GG型与阿司匹林抵抗密切相关。
Objective To investigate the relationship between cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) gene polymorphism and aspirin resistance. Methods 96 patients with ischemic stroke were divided into aspirin sensitive group 87 cases and aspirin non-sensitive group 9 cases. Two groups of patients received 100 mg of aspirin orally for at least one week. The platelet aggregation of the patients was measured by light nephelometry. The genotypes of COX-1 and COX-2 were detected by Sequenom Mass A RRAY i PLEX genotype analysis. The COX-1 and COX-2 gene polymorphism Correlation with aspirin. Results The proportion of past history of aspirin nonsensitive group was 66.7% (6/9) significantly higher than that of aspirin sensitive group (21.9%, 19/87) (P <0.05). There were significant differences in the distribution of COX-1 (rs1330344, rs3842788) between the two groups (P <0.05), but there was no significant difference in the distribution of COX-1 (rs5788) > 0.05). The polymorphisms of COX-1 (rs5788, rs1330344, rs3842788) and COX-2 (rs20417) alleles and genotype frequencies all conformed to Hardy-Weinberg equilibrium (P> 0.05). Conclusion COX-1 (rs3842788, rs1330344) GG is closely related to aspirin resistance.