Induction of type Ⅱ alveolar epithelial cells apoptosis in mouse by lipopolysaccharide does not req

来源 :Chinese Medical Journal | 被引量 : 0次 | 上传用户:qijing1
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
To examine whether lipopolysaccharide (LPS)-induced apoptosis correlates with TNF-α release by type Ⅱ alveolar epithelial cells (AEC Ⅱ), whether TNF-α knockout (TNF KO) abrogates the induction of apoptosis by LPS and whether TNF-α is sufficient to induce apoptosis in this cell type Methods AEC Ⅱ were isolated from wild type mice and TNF KO mice Cells were stimulated with LPS or recombinant murine TNF-α for 24 h TNF-α in culture supernatant was determined by ELISA following LPS stimulation Apoptosis was determined by the terminal deoxynucleotidyl transferase end-labeling (TUNEL) assay after treatment with either LPS or TNF-α Results LPS induced apoptosis in wild type AEC Ⅱ in a concentration-dependent manner LPS-induced AEC Ⅱ apoptosis was accompanied by an 11-fold increase (from 0 073±0 065 ng/ml in control to 0 94±0 14 ng/ml in 50 μg/ml of LPS, P<0 01) in TNF-α release However, increasing concentrations (5 or 25 ng/ml) of recombinant murine TNF-α failed to induce AEC Ⅱ apoptosis In addition, apoptosis did occur in AEC Ⅱ isolated from TNF KO mice following LPS stimulation Conclusions This study confirms that LPS induces TNF-α release and apoptosis in murine AEC Ⅱ in vitro Exogenous TNF-α failed to induce AEC Ⅱ apoptosis, and apoptosis occurred following LPS stimulation in cells lacking the ability to produce TNF-α Taken together, these results suggest that LPS-induced AEC Ⅱ apoptosis occurs by a TNF-α-independent mechanism To examine whether lipopolysaccharide (LPS) -induced apoptosis correlates with TNF-α release by type Ⅱ alveolar epithelial cells (AEC Ⅱ), whether TNF-α knockout (TNF KO) abrogates the induction of apoptosis by LPS and whether TNF-α is sufficient To induce apoptosis in this cell type AEC Ⅱ were isolated from wild type mice and TNF KO mice were stimulated with LPS or recombinant murine TNF-α for 24 h TNF-α in culture supernatant was determined by ELISA following LPS stimulation Apoptosis was determined by the terminal deoxynucleotidyl transferase end-labeling (TUNEL) assay after treatment with either LPS or TNF-α Results LPS induced apoptosis in wild type AEC Ⅱ in a concentration-dependent manner LPS-induced AEC Ⅱ apoptosis was accompanied by an 11-fold increase (from 0 073 ± 0 065 ng / ml in control to 0 94 ± 0 14 ng / ml in 50 μg / ml of LPS, P <0.01) in TNF-α release However, increasing concentrations (5 or 25 ng / ml ) of recombinant muri ne TNF-α failed to induce AEC Ⅱ apoptosis In addition, apoptosis did occur in AEC Ⅱ isolated from TNF KO mice following LPS stimulation Conclusions This study confirms that LPS induces TNF- α release and apoptosis in murine AEC Ⅱ in vitro Exogenous TNF-α failed to induce AEC Ⅱ apoptosis, and the growth of the LPS stimulation in cells lacking the ability to produce TNF-α Taken together, these results suggest that LPS-induced AEC II apoptosis occurs by a TNF-α-independent mechanism
其他文献
苏联有没有流行音乐?苏联党和政府以及其他艺术部门对流行音乐持什么样的态度?——这些都是我们颇感兴趣的问题。苏联没有“流行音乐”这种提法。有人说“游艺音乐”就相当
朝鲜第七届《四月之春国际友谊艺术节》于4月7日~18日在平壤举行。文化部组建中国艺术团一行45人前往参加。这届艺术节历时12天,规模盛大,有60多个国家和地区的77个艺术团参
借鉴国际经验改革我国税收管理体制浙江省国家税务局,浙江省地方税务局分税制,是当今世界各国普遍实行的财税体制,研究和比较各国的税收收入及其管理权限的划分方法,对改革完善我
目的:观察比较广枣中三种不同工艺提取物的抗心律失常作用,为找到一种抗心律失常作用较强的主要化学单体或简单复合物打下基础。方法:(1)乌头碱导致大鼠心律失常;(2)哇巴因导
根据日本1995年财政年度预算,汽车全部税收额为81344亿日元,占日本全部税收的9%(税收总收入为905 767亿日元)。汽车税收部分用于公路建设费用。 According to Japan’s 1995
2007年4月9日,《文汇报》出了个杂文专版,其中刊有《尊卑有别的无意识》一文。作者发现在词语运用中,隐藏着等级差别,比如,对领导用的词语是“尊重”,而“上级对下级,最多也
本期主题:艺术与消费文化  编者按:上一期关于“艺术消费文化专题”的文章从艺术与资本之间的关系探讨了消费时代的文化形态等问题,本期文章将从艺术消费经济伦理的角度探讨消费文化和雕塑艺术的发展之间的关系。    一、艺术按揭    资本的脚步离艺术越来越近了。在金融资本的热情注视下,将艺术品开发成为金融产品已成为资本市场的一个新的生长点,目前正被人们热议的艺术按揭就是其中之一。  艺术品按揭的方式,与
实行分税制体制,提高了各级财政增收的积极性,但由于各项管理没有跟上,地方财政收入的增长,仍然受到多种因素的制约。笔者通过深入调查,发现有六种主观因素制约了县(市)级有
关于地方税体系完善的几点思考孙智英随着社会主义市场经济体制的不断完善和财税体制改革的进一步深化,税收理论界与实际工作者已普遍认识到:要确立科学规范的财政分配关系,必须