Insect growth and development are precisely controlled by hormone homeosta-sis.The prothoracicotropic hormone (PTTH) receptor,Torso,is a member of the receptor tyrosine kinase family in insects.Activation of Torso by PTTH triggers biosynthesis and release of the steroid hormone in the prothoracic gland (PG).Although numbers of genes functioning in steroid hormone synthesis and metabolism have been identified in insects,the PTTH transduction pathway via its receptor Torso is poorly understood.In the cur-rent study,we describe a loss-of-function analysis of Torso in the silkworm,Bombyx mori,by targeted gene disruption using the transgenic CRISPR/Cas9 (clustered regularly inter-spaced short palindromic repeats/RNA-guided Cas9 nucleases) system.Depletion of B.mori Torso (BmTorso) did not eventually affect larval ecdysis and metamorphosis pro-cesses.Instead,BmTorso deficiency resulted in significant extension of developing time during larval and pupal stages with increased pupa and cocoon sizes.The ecdysteriod titers in the hemolymph of BmTorso mutants sharpy declined.Transcriptional levels of genes involved in ecdysone biosynthesis and ecdysteroid signaling pathways were signif-icantly reduced in BmTorso-deficient animals.Additionally,RNA-Seq analysis revealed that genes involved in the longevity pathway and protein processing in the endoplasmic reticulum pathway were affected after BmTorso deletion.These results indicate that Torso is critical for maintaining steroid hormone homeostasis in insects.