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目的探讨微管相关蛋白2(MAP-2)和巢蛋白在人胚胎脊髓发育阶段的分布规律及其表达意义。方法应用免疫组织化学法,检测第2、3、4月龄段共16例人胚胎脊髓前角、中央管及后角中MAP-2和巢蛋白的表达、分布状况。结果第2～4个月龄段,人胚胎脊髓内均可见巢蛋白表达阳性的神经纤维分布,随着胎龄的增大,脊髓前角处巢蛋白阳性表达的神经前体细胞和神经胶质细胞呈先增多再降低的趋势,在后角呈逐渐增高趋势。MAP-2在脊髓前后角神经细胞胞质中呈阳性表达,随胎龄增大而增强。结论 MAP-2和巢蛋白参与调控人胚胎脊髓神经细胞和神经胶质细胞的生长发育。
Objective To investigate the distribution of microtubule-associated protein 2 (MAP-2) and nestin in the development of human embryonic spinal cord and its significance. Methods The expression and distribution of MAP-2 and Nestin in the anterior horn, central canal and posterior horn of human fetus in the second, third and fourth month of age were detected by immunohistochemistry. Results In the second to fourth months of age, the distribution of nestin positive neurofibers was found in the human embryonic spinal cord. With the increase of gestational age, the positive neuronal precursor cells with nestin expression in the spinal cord anterior horn and glial The cells showed the trend of increasing first and then decreasing, and gradually increasing in the posterior horn. MAP-2 was positively expressed in the cytoplasm of neurons in the anterior and posterior horn of spinal cord, and increased with gestational age. Conclusion MAP-2 and Nestin are involved in the regulation of the growth and development of human embryonic spinal cord neurons and glial cells.